通过调解线粒体功能的作用,O-GlcNAc参与了老化卵子细胞的半变化
Chuwei Li1, Zhang Qian1, Hong Zhang1
1Department of Reproductive Medicine, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu, China.
Reproduction (Cambridge, England)
|October 15, 2024
概括
较高的O结合N-乙糖胺 (O-GlcNAc) 会导致卵细胞衰老,并损害线粒体功能. 降低老化卵细胞中的O-GlcNAc水平,可以提高其质量和介质成熟度.
科学领域:
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
- 老年学是一门学科.
背景情况:
- 由于母亲平均年龄的增加,女性生殖老龄化日益令人担忧.
- 卵细胞质量下降是母亲晚年的一个标志,但潜在的分子机制仍然不清楚.
- 与O相关的N-乙糖胺 (O-GlcNAc) 是一个关键的翻译后修饰,与与年龄相关的疾病有关.
研究的目的:
- 研究O-GlcNAc在卵细胞衰老中的作用.
- 为了确定O-GlcNAc修饰是否影响卵细胞质量和介质成熟.
主要方法:
- 在老化小鼠卵细胞中分析O-GlcNAc水平.
- 在卵细胞中评估介质成熟和线粒体功能.
- 在年轻卵细胞中,药理抑制和基因淘汰O-GlcNAcase (OGA).
- 在O-GlcNAc降低后,对线组织和卵细胞质量的评估.
主要成果:
- 老化小鼠卵细胞中的O-GlcNAc水平升高,与介质成熟失败和线粒体功能受损相关.
- 发现O-GlcNAc准了线粒体裂变蛋白与胺相关的蛋白质1,影响了线粒体的分布.
- 在年轻卵细胞中模仿与年龄相关的O-GlcNAc升高诱导了衰老的表型.
- 降低老化卵细胞中的O-GlcNAc水平恢复了螺丝组织,改善了卵细胞的整体质量.
结论:
- O-GlcNAc是介质成熟的重要调节者,在卵细胞衰老的进展中起着关键作用.
- 准O-GlcNAc修饰是一种潜在的策略,可以改善老年女性的卵细胞质量.
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