相关实验视频
Updated: Jun 12, 2026

10:12
Visualization of G3BP Stress Granules Dynamics in Live Primary Cells
Published on: May 21, 2014
18.3K
富含核酸的压力颗粒不仅仅是拥挤的冷凝物:一个定量拉曼成像研究
Ren Shibuya1, Shinji Kajimoto1,2, Hideyuki Yaginuma3,4
1Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
Analytical chemistry
|October 15, 2024
概括
细胞内液滴,或无膜有机体,通过液-液相分离 (LLPS) 形成. 这项研究量化了它们的化学成分,揭示了细胞功能和疾病至关重要的可变内部环境.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 生物化学 生物化学
背景情况:
- 无膜器官通过细胞内液态液相分离 (LLPS) 形成.
- 这些动态结构维持细胞平衡,但可以在神经退行性疾病中聚集.
- 量化滴滴成分是了解它们的功能和病理学的关键.
研究的目的:
- 在活细胞中由LLPS形成的应力颗粒 (SG) 中量化化学成分.
- 阐明细胞内液滴成分在细胞功能和应激反应中的作用.
- 为了研究LLPS滴滴环境和细胞应激类型之间的关系.
主要方法:
- 近红外光和拉曼成像用于化学分析.
- 定量拉曼强度分析,使用水作为内部标准,确定局部度.
- 相对于水的生物分子C-H频段强度量化了拥挤的环境.
主要成果:
- 核酸度在SG中比细胞质中高出20%以上;脂质度较低.
- 根据压力类型,SG的内部环境有所变化:在氧化压力下接近细胞质密度,在超的压力下稀少.
- 高度的核酸似乎对维持SG内部环境至关重要.
结论:
- 细胞内液滴 (无膜有机体) 具有可变的内部环境,并不总是高度凝结的.
- 液滴LLPS的成分是由细胞应激动态调节的.
- 了解这些化学动态对于细胞平衡和疾病研究至关重要.
相关概念视频
The Nucleolus
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
Additional Subnuclear Structures
The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals.
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles, paraspeckles, etc. These nuclear...
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles, paraspeckles, etc. These nuclear...

