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热冲击因子1形成核凝聚物,并在激活目标基因之前重组酵母基因组

Linda S Rubio¹, Suman Mohajan¹, David S Gross¹

¹Department of Biochemistry and Molecular Biology Louisiana State University Health Sciences Center, Shreveport, United States.

|October 15, 2024

概括

热冲击因子 (Hsf1) 快速重置热冲击反应 (HSR) 基因,并在乙醇压力下在酵母中形成凝结物,先进行转录激活. 这与热应激反应不同.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

3D基因组拓与昆虫和哺乳动物的转录状态有关.
基因组拓与其他真核生物的转录之间的关系尚不清楚.

研究的目的:

研究3D基因组拓在*Saccharomyces cerevisiae*在乙醇压力下调节热冲击反应 (HSR) 基因中的作用.
为了比较基因重新定位,凝聚物形成和转录激活的动力学,以回应乙醇与热应激.

主要方法:

结合近距离测试试验.
光显微镜的光学显微镜.
对Pol II占用率,染色体重塑和RNA表达的分析.

主要成果:

在乙醇压力下,HSR基因迅速重新定位并形成依赖热冲击因子 (Hsf1) 的基因间相互作用.
含有Hsf1的凝聚物迅速形成,但比Hsf1驱动的基因间相互作用持久得多.
转录激活,Pol II占用和染色质重塑在乙醇压力下比热压力更晚发生.
热应激会诱导快速,短暂的hsf1凝聚物,基因间相互作用和转录激活.

结论:

作为对蛋白质毒性压力 (乙醇和热量) 的反应,hsf1协调了3D基因组重组和凝结物形成.
基因组重组和转录激活的动力学在乙醇和热应激反应之间存在显著差异.
在乙醇应激中,HSF1驱动的基因组重新定位和凝聚物形成先于HSR基因的转录激活.

关键词:

三维基因组是3D的基因组.在 RNA Pol II 中.它们中的一种是S. cerevisiae.在这种情况下,染色染色素染色体是一种染色体.乙醇压力压力是一种压力.基因表达的基因表达方式热冲击反应的热冲击反应转录的凝聚物转录的凝聚物

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