β-caryophyllene对精子冷保存的作用
Emanuela Ribeiro Moura1, Marlon de Araújo Castelo Branco2, Yndyra Nayan Teixeira Carvalho Castelo Branco3
1Federal University of Piaui Post-Graduate Program in Tropical Zootechnics Teresina Piauí Brazil Post-Graduate Program in Tropical Zootechnics/Federal University of Piaui, Teresina, Piauí, Brazil.
JBRA assisted reproduction
|October 15, 2024
概括
补充β-caryophyllene (BC) 通过增强线粒体潜力和减少氧化应激,改善了冷保存的雄羊精液质量. 这种天然化合物有望改善Dorper公羊的精液保存.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 冰冷保存技术 冰冷保存技术
背景情况:
- 绵羊精液的冷保存对于遗传保护和人工授精至关重要.
- 氧化应激和线粒体功能障碍是影响解后精子质量的关键因素.
- β-caryophyllene (BC) 是一种具有已知的抗氧化特性的天然甲烯.
研究的目的:
- 评估在精液延长剂中补充β-caryophyllene (BC) 对多珀雄羊精子冷保存后质量的影响.
- 为了确定最佳的BC度以改善精液冷存活.
主要方法:
- 从六只多珀公羊收集精液,并将其冷保存在Tris蛋黄稀释剂中,其BC度各不相同 (0, 1.0, 2.0, 3.0mM).
- 解后的精液质量通过计算机辅助精液分析 (CASA),体和血完整性测试,线粒体膜潜力测试和热阻测试来评估.
- 氧化应激被评估使用铁酸反应物质 (TBARS) 试验.
主要成果:
- 补充β-caryophyllene并没有显著改变精子运动参数.
- 补充BC显著改善了冷保存的公羊精子中的线粒体膜潜力.
- TBARS测定表明,BC有效地减轻了解精液中的氧化应激.
结论:
- 在扩展器中补充β-caryophyllene会对冷保存的Dorper公羊精液的质量产生积极影响.
- BC增强了线粒体的功能,减少了氧化损伤,有助于在解后改善精液质量.
- 为了优化冷保存协议,需要进一步研究BC作为精液扩展剂的补充剂.
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