在使用不同烟草/尼古丁制品的人群中识别特定的血红蛋白吸附模式,通过非向GC-MS/MS分析
Fabian Pilz1, Therese Burkhardt1, Gerhard Scherer1
1ABF, Analytisch-Biologisches Forschungslabor GmbH, Semmelweisstr. 5, Planegg 82152, Germany.
Chemical research in toxicology
|October 15, 2024
概括
新的研究表明,在各种烟草和尼古丁产品的使用者中,血红蛋白 adduct 配置不同. 从烯二,烯胺和乙烯氧化物中提取的血红蛋白可以帮助验证吸烟和加热烟草产品的使用合规性.
科学领域:
- 毒理学 毒理学 毒理学
- 生物化学 生物化学
- 分析化学 分析化学
背景情况:
- 烟草烟雾中的电友化合物与DNA和血红蛋白等蛋白质形成添加物.
- 新的尼古丁和烟草制品被提议作为可燃香烟 (CC) 的不那么有害的替代品.
- 不同的烟草/尼古丁产品的使用者之间预计会有不同的血红蛋白 adduct 概况.
研究的目的:
- 开发和应用一种使用GC-MS/MS.的新型非标查血红蛋白 (Hb) 添加物的查策略.
- 分析和比较使用可燃香烟,电子香烟,加热烟草制品 (HTP),口服烟草,尼古丁替代疗法和非使用者的Hb adduct概况.
- 确定特定的Hb引证,并评估它们在验证产品使用和合规性方面的潜在有用性.
主要方法:
- 开发了一种使用GC-MS/MS的新型非向查策略,用于Hb adducts.
- 分析涉及经过修改的埃德曼降解后衍生N端氨酸添加物.
- 分析了来自代表各种烟草/尼古丁产品使用者和非使用者的临床研究队列的血液样本.
主要成果:
- 在研究的用户群体中观察到Hb adduct概况的显著差异.
- 确定的添加物包括甲基化/乙基化剂,乙烯氧化物,烯,烯胺,糖胺,和4-基甲.
- 与其他所有组相比,吸烟者表现出甲基化剂,乙烯氧化物,烯,烯胺和糖胺胺的添加剂水平较高. HTP使用者比非使用者具有更高的乙化,烯胺和糖胺添加物水平,但比吸烟者低.
结论:
- 各种烟草和尼古丁产品的使用者之间,Hb adduct 概况的特征有所不同.
- 由于其半衰期长,从烯,烯胺 (glycidamide) 和乙烯氧化物中提取的Hb,是验证吸烟和HTP使用研究合规性的有希望的生物标志物.
- 开发的非向方法提供了一个强大的工具,用于描述烟草/尼古丁产品用户对有害成分的暴露.
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