通过使用tetX携带菌株的生物增强来测序批量反应器中增强的四环素去除:效率和机制
Xiuli Chen1, Ying Zhu1, Shuang Yan1
1State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China.
Journal of hazardous materials
|October 15, 2024
概括
使用特定细菌菌株的生物增量有效地从废水中去除了四环素抗生素 (TCs). 这种方法还保护了基本的治疗过程,并增强了对抗生素降解的tetX基因的传播.
科学领域:
- 环境微生物学环境微生物学
- 废水处理技术 废水处理技术
- 抗生素耐药性的基因动态 抗生素耐药性的基因动态
背景情况:
- 四环素抗生素 (TCs) 是废水废水中发现的持续性环境污染物.
- 目前的废水处理系统在去除TC的有效性有限.
- 生物增强为增强TC去除提供了一个潜在的解决方案.
研究的目的:
- 为了评估使用Sphingobacterium sp.生物增强的有效性. 在测序批量反应堆 (SBR) 中用于TC去除的WM1和工程PUC-tetX菌株.
- 评估生物增量对TC降解和相关微生物群落的影响.
- 为了研究tetX基因在TC去除中的作用.
主要方法:
- 使用测序批量反应器 (SBR) 处理废水.
- 使用与野生类型 (Sphingobacterium sp. 和工程 (PUC-tetX) 细菌菌株.
- 量化了TC度,监测了脱和去除,分析了微生物群体的多样性,并使用元基因组分析跟踪了tetX基因丰度和转移.
主要成果:
- 生物增量减轻了TCs对脱和去除的抑制作用.
- 与PUC-tetX相比,WM1菌株表现出优异的TC从污泥中去除,并且具有更长的生命力.
- TC的添加和生物增量并没有显著改变整个微生物群体的多样性.
- 引入的细菌增加了tetX基因的丰富性,与增强的TC降解相关.
- 甲基因组分析显示,tetX基因转移到本地Chloroflexi细菌中,改善了TC去除.
结论:
- 生物增量是一种可行的策略,用于增强废水处理中的四环素抗生素去除.
- X基因在TC降解中起着至关重要的作用,其转移到土著细菌可以提高治疗效率.
- 生物增量可以保护基本的废水处理功能,同时解决抗生素污染问题.
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