高亲和度载体TaHAK1涉及耐受性和植物修复
Jin Liu1, Shuai-Bo Chen1, Ze-Hua Fan1
1National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou 450046, China.
Journal of hazardous materials
|October 15, 2024
概括
在大米中过度表达TaHAK1,通过改变Cs分布和改善 (K) 水平,提高对有毒 (Cs) 的耐受性. 这为植物修复和开发低碳水化合物米品种提供了战略.
科学领域:
- 植物生物学 植物生物学
- 环境科学 环境科学
- 生物化学 生物化学
背景情况:
- (Cs) 是一种有毒的性金属,影响人类健康.
- 植物高亲和运输体 (HAKs) 参与了Cs的吸收和运输,但监管机制尚不清楚.
- 了解由HAKs介导的Cs和 (K) 稳态对于管理Cs污染至关重要.
研究的目的:
- 研究TaHAK1在中的Cs吸收,运输和Cs/K稳态中的作用.
- 评估TaHAK1对植物修复和开发低Cs积累大米的潜力.
主要方法:
- 在大米中过度表达TaHAK1 (TaHAK1-OEs).
- 在Cs污染条件下分析TaHAK1-OEs中的Cs吸收,分布和K含量.
- 转录组和生物信息学分析以确定受影响的途径和基因.
主要成果:
- 太HAK1过度表达通过改变Cs分布,主要是在根细胞壁中,促进了幼苗的生长.
- 转录组分析揭示了与细胞壁生物合成和离子运输相关的基因的显著变化 (例如,XTHs,CSLEs,HAKs,ABCs).
- 塔哈克1-OE在各种组织,特别是谷物中显示出增强的Cs耐受性,降低了Cs积累,并增加了K含量.
结论:
- 在米中,TaHAK1在调节Cs吸收,转位和Cs/K稳态方面发挥着重要作用.
- TaHAK1是一种有希望的候选基因,可用于改造基因,以改善Cs植物修复和减少Cs在米中的积累.
- 这项研究为植物的Cs/K稳态和减轻Cs污染的策略提供了新的见解.
更多相关视频
10:08Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
Published on: December 9, 2022
2.1K
12:48Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays
Published on: February 19, 2013
10.5K
相关概念视频
01:17Reabsorption and Secretion in the Loop of Henle
1.0K
The thick ascending limb of the nephron loop has Na+–K+–2Cl− symporters in the apical membranes of its cells. These symporters simultaneously reclaim one sodium ion, one potassium ion, and two chloride ions from the tubular fluid. Sodium ions are actively transported into the interstitial fluid at the base and sides of the cell, diffusing into the vasa recta. Chloride ions move through leakage channels in the basolateral membrane into the interstitial fluid and then into the...
1.0K
01:14Active Transport
504
Active transport is a critical biological process that allows cells to move solutes against an electrochemical gradient. This process requires direct energy input and is characterized by its selectivity, saturability, and susceptibility to competitive inhibition.
Primary active transporters, like Na+, K+ and -ATPase, directly utilize ATP to move ions across the membrane. These transporters play significant roles in various physiological processes. For instance, Na+, K+ and -ATPase maintain...
Primary active transporters, like Na+, K+ and -ATPase, directly utilize ATP to move ions across the membrane. These transporters play significant roles in various physiological processes. For instance, Na+, K+ and -ATPase maintain...
504
01:23Transcellular Transport of Solutes
3.5K
Transcellular transport of solutes is the movement of substances like monosaccharides and amino acids through polarized cells. This transport mechanism is primarily seen in epithelial and endothelial cells aided by membrane transport proteins such as channels and transporters. The tight junctions between these cells confine the membrane proteins to the two sides of the cell. The epithelial cells have distinct apical and basolateral domains. In contrast, the endothelial cells show the luminal...
3.5K
01:20Tonicity in Plants
30.2K
Plant cells maintain appropriate osmotic balance in extreme conditions. For instance, plants in dry environments store water in vacuoles, limit the opening of their stoma, and have thick, waxy cuticles to prevent unnecessary water loss. Some species of plants that live in salty environments store salt in their roots. As a result, water osmosis occurs in the root from the surrounding soil.
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...
30.2K
01:28Antihypertensive Drugs: Potassium-Sparing Diuretics
469
Liddle syndrome is a genetically inherited form of hypertension characterized by the overactivity of epithelial sodium channels in the nephron, the functional unit of the kidney. This heightened activity leads to increased sodium reabsorption and excessive excretion of potassium. To counteract this, potassium-sparing diuretics such as amiloride are used. They function by blocking these sodium channels, thereby reducing the influx of sodium into the epithelial cells and minimizing the loss of...
469
02:02Responses to Salt Stress
13.0K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.0K