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增强的内质网膜应激信号破坏了猪肉细胞功能,以应对双A暴露

Ting Hu¹, Jiaxi Zhang¹, Yuxuan Wei¹

¹Animal Science and Technology College, Beijing University of Agriculture, Beijing, 102208, China.

Journal of environmental management

|October 15, 2024

概括

在养猪场的双甲 (BPA) 污染会损害猪精子的运动性和塞尔托利 (ST) 细胞的功能. 乙A引发了内分泌网膜应激,氧化应激和炎症,导致细胞亡和生殖能力下降.

科学领域:

环境科学环境科学
生殖生物学生殖生物学
毒理学毒理学毒理学

背景情况:

双A (BPA) 是一种在食品,水和牲畜环境中广泛存在的污染物.
暴露于BPA会对男性生殖健康产生负面影响,特别是对于精子发育至关重要的塞尔托利 (ST) 细胞.

研究的目的:

调查猪业中BPA的流行情况.
评估BPA对野猪精子运动和猪ST细胞功能的影响.
阐明BPA生殖毒性背后的分子机制.

主要方法:

分析了来自130项研究的全球BPA污染数据.
在猪料和血清中测量BPA度.
在实验室中,野猪精子和猪ST细胞暴露于BPA.
转录组分析以确定分子通路.
使用4-PBA抑制内细胞网膜应激 (ERS).

主要成果:

在猪料 (9.747.9μg/kg) 和血清 (55.175.6 ng/L) 中量化了BPA度.
较高的BPA水平与精子活力和运动率的降低相关.
暴露于BPA会损害野猪精子的运动性和ST细胞活动.
BPA上调了与ERS相关的基因 (PERK,IRE1α,ATF6) 并通过NF-κB触发了氧化应激和炎症.
BPA诱导了ST细胞亡,通过使用4-PBA抑制ESR减弱了这种作用.

结论:

猪业中的BPA污染对猪的生殖健康构成重大风险.
持续激活ERS,氧化应激和炎症是猪ST细胞中BPA毒性的关键机制.
针对ESR可能提供一种治疗策略,以减轻BPA引起的生殖损伤.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA BPA 在此之前,ERS 炎症反应是一种炎症反应.在ST细胞中,ST细胞是精子动物的运动性.

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