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重新冷扩展器对马精子质量和胚胎生产在细胞内质精子注射后的作用

Leonardo F C Brito¹, Matheus R Felix¹, Renata L Linardi¹

¹Department of Clinical Studies - New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA, 19348, USA.

|October 15, 2024

概括

重新冷马精液以进行细胞体内精子注射 (ICSI) 是可行的. 使用低蛋黄/牛奶/糖醇扩展剂进行重新冷,可以改善胚胎生产,而不是用胺扩展剂.

科学领域:

生殖生物学生殖生物学
动物科学动物科学
辅助生殖技术是一种辅助生殖技术.

背景情况:

内细胞质精子注射 (ICSI) 对于辅助生殖至关重要,特别是有限的精子.
重新冷精液可以增加ICSI的可用性,但其对结果的影响需要调查.
扩展剂在ICSI的精子重新结中的作用尚不清楚.

研究的目的:

为了评估不同重新结扩展器对马精子质量的影响.
评估重新结扩展器在ICSI后对胚胎生产的影响.
为了确定最佳的扩展器重新结马精液的ICSI.

主要方法:

马的精液最初被冷在一个低蛋黄/甘油/胺扩展剂中.
解后的精液被重新扩展并使用三个不同的扩展剂重新结 (扩展器1:低蛋黄/甘油/胺基;扩展器2:高蛋黄/甘油;扩展器3:低蛋黄/牛奶/甘油).
在ICSI后的精子质量,切割率和胚胎细胞发育率与冷后的精子进行了比较.

主要成果:

与经过一次冷的精液相比,使用扩展器1 (含胺) 进行重新冷导致了较低的裂变和胚胎囊率.
使用扩展器2或扩展器3重新结并没有显著降低胚胎生产率.
在扩展器1中重新结的精子显示出高运动性和活力,表明这些指标不足以评估ICSI适用性.

结论:

最初的冷扩展器对ICSI结果的影响最小.
再缩扩展器通过ICSI显著影响马胚胎的生产.
低蛋黄/牛奶/糖扩展剂是有效的重新结马精液的ICSI.
在为ICSI重新冷马精液时,应避免使用扩展器中的胺.

关键词:

马马的马,就是马马.美国国务院调查局 (ICSI)在细胞体内注射精子.精子精子精子精子精子精子精子马的马匹马的马匹

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