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斑马鱼中瓦尔酸对心血管发育的危害
Yuqing Lei1, Yingying Liu2, Wenpeng Xie3
1Medical Research Center, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou 350013, China; Fujian Key Laboratory of Women and Children's Critical Diseases Research, Fujian Maternity and Child Health Hospital, Fuzhou, Fujian 350001, China.
Ecotoxicology and environmental safety
|October 15, 2024
概括
瓦尔酸 (VPA) 在斑马鱼胚胎中引起发育迟缓和心血管形. 这种表观遗传药物带来潜在的环境风险,特别是在怀孕早期.
科学领域:
- 发展性毒理学 发展性毒理学
- 心血管研究的心血管研究.
- 环境健康 环境健康
背景情况:
- 酸 (VPA) 广泛用于和癌症,但已知有致病作用.
- VPA对循环系统发展的影响尚不清楚.
- 临床使用VPA引发了对环境危害的担忧.
研究的目的:
- 调查VPA暴露对斑马鱼心血管发育的影响.
- 阐明VPA诱导心脏毒性背后的分子机制.
- 在早期开发过程中评估VPA的潜在环境风险.
主要方法:
- 斑马鱼胚胎暴露于从受精后3小时 (hpf) 到受精后5天 (dpf) 的不同VPA度.
- 评估了心血管发育,运动和反应时间.
- 分析了心肌反应性氧物种 (ROS),亡以及与心血管发育,抗氧化反应和亡相关的基因表达.
主要成果:
- 暴露于VPA导致了剂量和时间依赖的发育延迟,包括心血管形.
- 由于线粒体功能障碍,在心脏中观察到ROS水平升高和细胞亡.
- 观察到心血管发育和抗氧化基因的下调,以及与亡相关的基因的上调.
结论:
- 暴露于VPA显著损害斑马鱼胚胎的心血管发育.
- 在怀孕早期,VPA对心血管健康构成潜在风险.
- 本研究强调了与VPA相关的环境危害及其对发育循环系统的影响.
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