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禽类三角冠状病毒编码与融合相关的小跨膜蛋白质,可以诱导合成细胞形成

Kylie Sartalamacchia¹, Monique S Porter², Vanesa Veletanlic¹

¹Department of Pediatrics, Vanderbilt University Medical Center, 2200 Children's Way, Suite 2404, Nashville, TN, 37232, USA.

|October 15, 2024

概括

研究人员在禽类三角冠状病毒中确定了与融合相关的小跨膜 (FAST) 蛋白质. 这些非结构性病毒蛋白可以诱导细胞细胞融合,这是病毒传播的关键步骤.

科学领域:

病毒学病毒学
分子生物学分子生物学
结构生物学结构生物学

背景情况:

融合相关的小跨膜 (FAST) 蛋白质是已知的非结构性病毒蛋白质,它们调解细胞-细胞融合.
这些蛋白质以前在双链RNA病毒中被发现,具有特征性的结构特征:一个乙基化N终端的ectodomain,一个中心的跨膜域和一个具有多基区域的C终端内域.

研究的目的:

在禽类三角冠状病毒的辅助蛋白中识别假定的FAST蛋白.
为了研究这些已识别的禽类冠状病毒蛋白的细胞融合能力.

主要方法:

用序列同性学和蛋白质基因预测来识别禽类三角冠状病毒中潜在的FAST蛋白.
使用特定的禽类冠状病毒蛋白进行过渡性表达试验 (冠状病毒NS7b,普通冠状病毒NS7a,夜冠状病毒NS7b).
通过检测不同细胞系 (灵长类上皮细胞,纤维细胞,胚胎纤维细胞) 中的合成细胞形成,并通过评估N端添加的效果来评估融合活性.

主要成果:

来自松鼠冠状病毒NS7b和常见松鼠冠状病毒NS7a的辅助蛋白被确定为假定的FAST蛋白.
这两种蛋白质的短暂表达在灵长类脏上皮细胞和纤维细胞中诱导了显著的细胞-细胞融合 (合成细胞形成).
融合活动取决于特定的病毒蛋白和细胞类型,夜冠状病毒NS7b没有显示融合活动,并通过N端FLAG添加取消了融合.

结论:

多种鸟类三角冠状病毒编码非结构性蛋白质,它们的功能类似于已知的FAST蛋白质,调解细胞-细胞融合.
这些发现突出了不同病毒家族细胞融合的保存机制,并表明这些蛋白质在deltacoronavirus生物学中的潜在作用.
需要进行进一步的研究,以阐明这些类似FAST的蛋白质对deltacoronavirus生命周期和发病的精确贡献.

关键词:

新冠病毒新冠病毒新冠病毒.德尔塔冠状病毒病毒这是一种快速的蛋白质.这就是Fusogen.复原病毒是复原病毒.罗塔病毒 (Rotavirus) 是一种病毒.这里是 Syncytia 的位置.

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