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饮食中的二氧化纳米颗粒通过扰乱肠道干细胞的功能,损害肠道上皮再生

Xiu Wang¹, Kai Zou¹, Yu Xiong²

¹School of Advanced Materials Engineering, Jiaxing Nanhu University, Jiaxing, 314000, China; Jiaxing Key Laboratory for Research and Application of Green and Low-carbon Advanced Materials, Jiaxing, 314000, China.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

|October 15, 2024

概括

饮食中的二氧化纳米颗粒 (TiO2 NPs) 恶化肠道炎症并损害肠道干细胞再生,与微粒不同. 这突出了TiO2NP的存在.

科学领域:

胃肠道学和毒理学
干细胞生物学干细胞生物学
纳米技术纳米技术

背景情况:

肠道干细胞 (ISC) 对肠道健康至关重要,对光质物质非常敏感.
关于食品添加剂,如二氧化 (TiO2) 对ISC的影响的数据有限.
食纳米颗粒 (NP) 由于其尺寸和特性,可能会带来独特的风险.

研究的目的:

研究二氧化纳米颗粒 (TiO2NP) 与微粒 (TiO2MP) 对肠道健康和ISCs的影响.
阐明TiO2NP在肠炎期间影响肠干细胞功能和再生的机制.
评估颗粒大小对TiO2作为食品添加剂毒性的作用.

主要方法:

利用酸 (DSS) 诱导的肠炎的小鼠模型.
向小鼠口服 1% (w/w) TiO2 NP 或 TiO2 MP.
使用3D肠道器官模型 (ex vivo和in vitro) 研究TiO2 NP对20μg/mL的ISC的影响.
分析了上皮细胞增殖,细胞死亡,循环率和Wnt信号通路激活 (β-catenin核转位).

主要成果:

TiO2 NPs,但不是TiO2 MPs,在小鼠中加剧了DSS诱导的肠炎.
暴露于TiO2 NP恶化了ISC介导的肠上皮再生,通过减少增殖和循环,同时增加细胞死亡.
在有机体模型中,TiO2 NPs损害了ISC功能和命运规范,通过抑制β-catenin核转移来阻碍Wnt信号传输.

结论:

饮食中的TiO2NP会对肠道健康和ISC功能产生不利影响,加剧DSS诱导的肠炎.
2NP的有害影响与ISC介导的上皮再生受损和Wnt信号中断有关.
颗粒大小是TiO2对肠道健康影响的关键因素,这表明需要在食品添加剂应用中进行优化.

关键词:

细胞命运规范细胞命运规范肠道有机物肠道有机物肠道干细胞肠道干细胞二氧化纳米颗粒的二氧化.没有信号发送.

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