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体显示器抗体库的选择:扫描程序

Haiyong Peng¹, Christoph Rader²

¹Department of Immunology and Microbiology, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, University of Florida, Jupiter, Florida 33458, USA.

Cold Spring Harbor protocols

|October 15, 2024

概括

体显示使抗体生成和进化成为可能. 本协议详细介绍了使用各种抗原格式的抗体库的选择策略,包括固定,基于溶液和细胞表面的标.

科学领域:

生物技术是生物技术.
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

体显示是一种产生和演变单克隆抗体的强大技术.
抗体库对于抗体的发现和开发至关重要.

研究的目的:

用 pComb3 phagemid 家族生成的抗体库的选择策略.
提供一种协议,用于对抗体库进行针对不同抗原格式的扫描.

主要方法:

使用菌体显示器选择抗体库.
用于固定抗原的Panning程序.
用于溶液中的生物化抗原的制程序.
对于细胞表面抗原的扫描程序.

主要成果:

证明了针对各种抗原类型的抗体库的成功选择.
提供了一种与不同抗体格式 (例如,Fab) 兼容的多功能协议.

结论:

描述的菌体显示选择策略对于抗体库查是有效的.
该协议可为各种应用提供单克隆抗体的生成和优化.

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