优化Exopolysaccharide生产的细菌使用坏的无花果和葡萄.
Shymaa R Bashandy1, Mohamed Hemida Abd-Alla2, Esraa A Mohammed2
1Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt. Bashandy@aun.edu.eg.
Current microbiology
|October 15, 2024
概括
这项研究利用 Bacillus subtilis 优化了外聚糖 (EPS) 产量,并发现腐烂的葡萄和无花果提取物是有效的,具有成本效益的基质. 补充这些特定营养素显著提高了EPS产量,促进了可持续的生物聚合物生产.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 可持续的生物处理
背景情况:
- 外聚糖 (EPS) 是有价值的生物聚合物,具有多种应用.
- 细菌细菌是已知的EPSs的生产者.
- 利用废物作为生物聚合物生产的基材,为经济和环境带来了好处.
研究的目的:
- 为了增强Bacillus subtilis ES (OR501464) 的外聚糖 (EPS) 生产.
- 评估腐烂的葡萄和无花果提取物作为EPS合成的成本效益高的基板.
- 优化影响EPS生产的营养因素.
主要方法:
- 隔离和识别高EPS产生的细菌菌株.
- 优化生产介质的成分 (糖,NaNO3,Na2SO4,NaCl),温度和pH值.
- 评估EPS生产使用腐烂的葡萄和无花果提取物与不同的营养补充剂.
- 使用GC-MS和FTIR光谱学对EPS的部分表征.
主要成果:
- 细菌细菌ES (OR501464) 被确定为一个高产量的EPS生产者.
- 最佳生产介质的产量为4.7 g/L EPS,含有4%的糖,0.1%的NaNO3,0.002%的Na2SO4和2%的NaCl,在30°C和pH值9.0的情况下.
- 用2%的NaCl补充的腐烂葡萄提取物增加了EPS产量,达到4.357 mg/mL.
- 用0.2g/L的Na2SO4和1g/L的NaNO3增强EPS生产来补充腐烂的无花果或葡萄提取物.
结论:
- 腐烂的无花果和葡萄是细菌EPS生产的可行,可持续的基板.
- 营养优化显著提高了这些废物材料的EPS产量.
- 这项研究支持环保生物处理,以产生有价值的生物聚合物.
相关概念视频
Biosynthesis of Polysaccharides
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
Microbes in Food Production
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Microbes in the Production of Fermented Foods
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Production of Alcohol
Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...


