相关实验视频

Updated: Jun 10, 2025

Improved Methodology for Studying Postnatal Osteogenesis via Intramembranous Ossification in a Murine Bone Marrow Injury Model

Improved Methodology for Studying Postnatal Osteogenesis via Intramembranous Ossification in a Murine Bone Marrow Injury Model

Published on: February 7, 2025

在PDGF-BB治疗骨髓衍生中介细胞干细胞后,动态染色质可访问性和转录组变化

Sheng Liu¹, Xiaona Chu¹, Jill L Reiter¹

¹Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN, 46202, USA.

|October 15, 2024

概括

血小板衍生生长因子 (PDGF) -BB通过改变染色质可访问性来增强介质干细胞 (MSC) 的增殖和分化. 捐赠者变异影响分化,PDGF-BB可能通过外体促进细胞间通信.

科学领域:

干细胞生物学干细胞生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

介酶干细胞 (MSC) 是具有治疗潜力的多能细胞,但面临着低移植等挑战.
血小板衍生生长因子 (PDGF) -BB正在研究,以提高MSC的治疗疗效.
了解PDGF-BB对MSC基因表达和染色体的影响至关重要.

研究的目的:

研究PDGF-BB对人类初级MSCs的基因表达特征的影响.
分析PDGF-BB对MSCs染色质可访问性景观的影响.
阐明了PDGF-BB介导的MSC调制背后的分子机制.

主要方法:

对人类初级MSCs进行了RNA测序,有或没有PDGF-BB刺激.
进行了染色素可访问性分析,以评估监管区域的变化.
采用了转录和表观基因组数据的综合分析.

主要成果:

PDGF-BB治疗改变了染色质可访问性格局,影响了基因激活和抑制.
观察到AP-1转录因子家族成员 (TEAD,CEBP,RUNX2) 的变化.
PDGF-BB促进了MSC的扩散和分化成骨质母细胞,脂肪细胞和肌细胞,具有依赖于捐赠者的变异.

结论:

通过AP-1转录因子,PDGF-BB诱导MSC的增殖和分化.
捐赠者特定的差异显著影响MSC差异化程度.
对外体相关基因的丰富表明PDGF-BB在细胞间通信中的作用.

关键词:

染色体的可访问性染色体的可访问性介质细胞干细胞介质细胞干细胞血小板衍生生长因子 (PDGF) -BBBB

更多相关视频

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

Published on: June 1, 2018

相关概念视频

Chromatin Modification in iPS Cells

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...