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脂质和皮质类固醇生物标志物在双酸盐的影响下

Kathy Tou¹, Adam Cawley², Glenys Noble³

¹Centre for Forensic Science, University of Technology Sydney, Sydney, New South Wales, Australia.

Drug testing and analysis

|October 15, 2024

概括

在马中检测双酸盐 (BPs) 很困难,因为它们的排泄速度很快. 这项研究发现,龙酸 (ZA) 在8小时后无法检测,而龙酸 (TA) 持续28天,有助于检测策略.

科学领域:

马体育医学马体育医学
分析化学分析化学
药理动力学药理动力学

背景情况:

双酸盐 (BPs) 在马匹运动员中被使用,由于其止痛和骨质改变作用,引起了福利方面的担忧.
在生物样本中检测BP是具有挑战性的,因为消除和回收率可变.
需要补充检测方法来识别BP在赛马中的滥用.

研究的目的:

在马中研究酸 (zoledronic acid,ZA) 和非酸 (tiludronic acid,TA) 双酸盐的血消除.
分析内源性eicosanoids和皮质类固醇的变化作为潜在的生物标志物.
开发改进的分析方法,以检测马匹运动员滥用双酸盐.

主要方法:

静脉注射ZA和TA给8匹纯种马,用11个月的洗期.
在两步固相提取和衍生后,使用液体染色学-并联质谱法 (LC-MS/MS) 量化BP.
通过液体染色学高分辨率质谱法 (LC-HRMS) 分析了内源生物标志物,在蛋白质沉和SPE后获得数据独立.

主要成果:

给药后8小时内无法检测到佐乐龙酸 (ZA).
提卢德龙酸 (TA) 在服用后28天内被检测到.
观察到eicosanoid和皮质类固醇生物标志物的变化正在验证中.

结论:

ZA和TA的差异性消除概况需要定制的检测策略.
根据其药理动力学特性,LC-MS/MS提供了针对特定BP的有效量化.
已识别的内源生物标志物显示出有望提高对马类血中双酸盐滥用的追溯检测.

关键词:

在反兴奋剂的反兴奋剂.生物标志物生物标志物双酸盐是一种双酸盐.脂质脂质脂质是一种血等离子体是什么?

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