提取物清理与目前用于分散固相提取的吸附剂的系统比较
Michelle Peter1, Christoph Müller1
1Department of Pharmacy, Center for Drug Research, Ludwig-Maximilians-Universität München, 81377 Munich, Germany.
这项研究比较了分散固相提取 (dSPE) 吸附剂用于多残留分析. PSA吸附剂表现出最好的整体性能,在各种食品矩阵中平衡清理效率与分析物回收.
科学领域:
- 分析化学 分析化学
- 环境化学环境化学
- 食品化学 食品化学
背景情况:
- 分散固体相提取 (dSPE) 对于多重残留分析至关重要,净化提取物以防止仪器污染并提高方法性能.
- 在dSPE过程中过度净化可能会通过吸附导致分析物损失,需要仔细选择吸附剂.
- 评估既有和新的dSPE吸附剂对于优化分析方法至关重要.
研究的目的:
- 系统地比较各种dSPE吸附剂的清理能力和分析回收.
- 为了评估PSA,GCB,C18,基,基托,MWCNT和Z-Sep®吸附剂的性能.
- 确定不同吸附剂对各种食品矩阵中98种分析物的回收的影响.
主要方法:
- 系统地比较dSPE吸附剂,包括PSA,GCB,C18,基,基托,MWCNT和Z-Sep®.
- 通过视觉检查,紫外线和GC-MS测量来评估清理能力.
- 用GC-MS/MS在菜,子,梨,鱼和牛肝矩阵中的98个化合物的分析物回收率的确定.
主要成果:
- 在紫外线和GC-MS测量中,Z-Sep®表现出最高的清洁能力,在紫外线和GC-MS测量中,矩阵组件的中位数减少了50%.
- 多壁碳纳米管 (MWCNTs) 显著影响了分析物回收,14种分析物回收率低于70%.
- PSA 吸附剂提供了最佳的整体性能,平衡了有效的清洁与最小的分析物损失.
结论:
- 在多残留物分析中,PSA是获得最佳结果的推吸附剂,在矩阵去除和分析物回收之间提供了平衡.
- 吸附剂的选择对复杂食品样本的净化效率和定量分析的准确性都具有重要影响.
- 像Z-Sep®这样的新型吸附剂对矩阵减少有希望,但必须仔细管理潜在的分析物损失.
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