相关实验视频

Updated: Jun 10, 2025

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Published on: November 16, 2018

大麻诱导的外体对细尘引起的牙周损伤的功能

Eunhee Kim¹, Yoonjin Park², Mihae Yun³

¹Department of Food Science and Biotechnology, Andong National University, Andong 36729, Republic of Korea.

International journal of molecular sciences

|October 16, 2024

概括

成熟的麻茎提取物诱导外体 (MSEIEs) 通过减少炎症和促进干细胞分化来保护口腔细胞免受细尘的影响. 这些外体具有前景,可作为各种行业的功能生物材料.

科学领域:

口腔健康研究研究口腔健康研究
生物材料科学生物材料科学
异构体生物学异构体生物学

背景情况:

微尘暴露与各种系统性健康问题有关,但其对口腔健康的影响还未得到充分研究.
牙周病影响支牙的组织,是严重的公共卫生问题.

研究的目的:

调查成熟的麻茎提取物诱导外体 (MSEIEs) 对细尘诱导的牙周细胞损伤的保护作用.
探索MSEIE保护行动的潜在机制.

主要方法:

使用了微RNA分析,聚合酶链反应 (PCR),流细胞测量,免疫细胞化学,酶相关免疫吸收试验 (ELISA) 和阿利沙林O染色.
牙周带干细胞 (PDLSCs) 被暴露在微尘中,并用MSEIE处理.

主要成果:

MSEIE上调特定的微RNA (hsa-miR-122-5p,hsa-miR-1301-3p,hsa-let-7e-5p) 参与生物功能.
MSEIE抑制了炎症基因表达,同时促进了抗炎基因.
MSEIE促进了PDLSC分化为骨质母细胞,并调节了关键蛋白表达 (LL-37,MCP-1).

结论:

MSEIE显示出对牙周细胞的显著保护作用,防止接触细尘.
作为药品,化品和食品应用的功能生物材料,MSEE具有潜力.
对MSEIE的进一步研究可能会导致口腔健康的新疗法策略.

关键词:

在PM10中,PM10是什么?大麻大麻大麻大麻大麻大麻大麻大麻外基因组是外基因组中的一个.牙周病是一种牙周病.牙周带干细胞的干细胞

更多相关视频

Therapeutic Effectiveness of a Dietary Supplement for Management of Halitosis in Dogs

Therapeutic Effectiveness of a Dietary Supplement for Management of Halitosis in Dogs

Published on: July 6, 2015

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

相关概念视频

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

Role of Matrix Metalloproteases in Degradation of ECM

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...