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脑脊液和外围血液淋巴单细胞单细胞转录在一个急性感染艾滋病毒的多发性硬化症患者身上

Carmela Pinnetti¹, Gabriella Rozera², Francesco Messina³

¹Clinical and Research Infectious Department, National Institute for Infectious Diseases Lazzaro Spallanzani-IRCCS, 00149 Rome, Italy.

International journal of molecular sciences

|October 16, 2024

概括

单细胞转录组学揭示了多发性硬化症和急性艾滋病毒感染患者的神经退行性基因特征. 这项研究强调了单细胞分析在复杂的神经疾病中的潜力.

科学领域:

神经免疫学神经免疫学
病毒学病毒学
基因组学就是基因组学.

背景情况:

多发性硬化症 (MS) 和人类免疫缺陷病毒 (HIV) 感染都会影响中枢神经系统.
调查共感染提供了对复杂疾病机制的洞察.

研究的目的:

通过单细胞转录学,研究一种独特的MS病例与急性HIV感染中的神经退行信号.
探索单细胞RNA测序 (scRNA-seq) 在剖析复杂的神经病理方面的实用性.

主要方法:

单细胞RNA测序 (scRNA-seq) 在脑脊液 (CSF) 和外周血液单核细胞 (PBMC) 上进行.
数据分析涉及Cell Ranger软件和定制生物信息管道,包括使用公共MS scRNA-seq数据集进行无监督的集群.
在特定的T细胞群体内确定了HIV-1转录.

主要成果:

分析产生了1446个CSF和4647个PBMC单细胞转录组.
与PBMC相比,CSF样本显示有丰富的T细胞,血细胞和血细胞 dendritic细胞 (pDCs).
在四个群体中观察到上调的微质神经退行性基因表达,其中两个包含患者的数据.
在CSF中的CD4中央记忆T细胞中检测到HIV-1转录 (gag-pol,vpu,env).

结论:

单细胞转录学在患有同时发生的MS和急性HIV的患者中确定了神经退行性基因表达模式.
该研究无法最终将观察到的神经退行症归因于MS或HIV.
这项研究表明,scRNA-seq可用于研究复杂的神经疾病的高分辨率.

关键词:

在传染病中免疫调节失调.传染病原体和免疫反应之间的相互作用.

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