蛋白质,微RNA在细胞外囊中的表达和大米种的活力之间的关系
Rouxian Wu1, Bingxian Chen1, Junting Jia1
1Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
International journal of molecular sciences
|October 16, 2024
概括
植物细胞外囊泡通过影响细胞壁合成和代谢途径来调节种子活力. 这项研究确定了这些囊泡中的关键蛋白质和miRNAs,为种子生存能力和农业应用提供了新的见解.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 植物细胞外囊泡 (EVs) 是由植物细胞释放的脂质双层限定的颗粒,含有脂质,RNA和蛋白质.
- 种子活力对于农业生产和生殖质保存至关重要.
- EVs调解跨物种通信,并具有抗病原体和抗衰老等功能,但它们在种子活力中的作用尚未被探索.
研究的目的:
- 研究植物细胞外囊泡对大米种子活力的潜在影响.
- 识别EV中涉及影响种子生存能力的蛋白质和miRNAs的调控机制.
主要方法:
- 从不同发芽百分比的水种子中提取细胞外囊泡,内源蛋白和RNA.
- 蛋白质的定性鉴定和微RNAs (miRNAs) 的差异分析.
- 相关性分析将EV组件和生物过程与种子活力联系起来.
主要成果:
- 四个miRNA家族 (osa-miR164, osa-miR168, osa-miR166, osa-miR159) 显示出显著的差异性表达.
- 电动体可能调节种子细胞壁合成,氧酸和三碳酸循环,脂质转移和线粒体质量控制.
- 诸如库宾,脂酶D,化脱酶,热冲击蛋白 (BiP1,BiP2),PDI,硫素,calnexin,calreticulin和谷氨转移酶等蛋白质与种子活力有显著的相关性.
结论:
- 植物细胞外囊泡在调节大米种子活力方面发挥着作用.
- 特定的miRNA和EV中的蛋白质是种子生存能力的关键媒介.
- 这项研究为通过基于EV的机制了解和改善种子活力开辟了新的途径.
相关概念视频
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:51mRNA Stability and Gene Expression
2.8K
2.8K
01:23Regulation of Expression at Multiple Steps
875
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
875
02:24Regulation of Expression Occurs at Multiple Steps
22.5K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.5K
02:31Nuclear Export of mRNA
7.6K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.6K
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K