扩展的基因调控网络揭示了Cordyceps militaris中潜在的光响应转录因子和向基因
Paradee Buradam1,2, Roypim Thananusak3, Mattheos Koffas4,5
1Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
International journal of molecular sciences
|October 16, 2024
概括
这项研究确定了Cordyceps militaris中的关键光响应基因和转录因子 (TF),揭示了它们在真菌生长和代谢物产生中的关键作用. 这些发现建立了一个功能网络,用于理解C. militaris.中的光信号.
科学领域:
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 科迪塞普斯军用菌被认为是生物活性化合物,如cordycepin.
- 的生长和新陈代谢受光信号通路的影响.
- 在C. militaris中,对光敏感的基因调节是不太了解的.
研究的目的:
- 在C. militaris.中确定对光敏感的基因和转录因子 (TF).
- 为C. militaris.重建一个扩展的基因调节网络 (eGRN).
- 研究新型TF在光信号传输中的潜在作用.
主要方法:
- 综合性转录组分析比较光和黑暗条件.
- 使用基于interolog和基于homolog的方法重建一个eGRN.
- 分子对接分析以评估TF蛋白相互作用.
主要成果:
- 在光敏感子网络中确定了五个关键TF和十个受调节的基因.
- 突出了这些基因/TFs在C. militaris生长和二次代谢产物的潜在重要性.
- 发现了两种新的TF (CCM_05727,CCM_06992) 与CmWC-1具有强烈的结合亲和力.
结论:
- 建立了一个功能互动网络,用于C. militaris.的全球转录调节.
- 提供了对这种真菌对光敏感基因调节的机制的见解.
- 奠定了未来关于真菌光信号和代谢物产生研究的基础.
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