葡萄乳酶基因的鉴定及其在二次代谢物合成中的潜在作用
Hao Wang1, Haixia Zhong1, Fuchun Zhang1
1The State Key Laboratory of Genetic Improvement and Germplasm Innovation of Crop Resistance in Arid Desert Regions, Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables, Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China.
International journal of molecular sciences
|October 16, 2024
概括
研究人员确定了115个葡萄乳酶 (VviLAC) 基因,揭示了它们在果发育和代谢物合成中的作用. 关键的VviLACs会影响水果的颜色,并受到像WRKY和ERF这样的转录因子的调节.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 生物化学 生物化学
背景情况:
- 乳糖酶 (含铜的氧化还原酶) 对于植物的二次代谢物生物合成至关重要.
- 乳糖酶活动通过影响代谢物合成和积累,影响植物生长,发育和抗压能力.
研究的目的:
- 为了识别葡萄葡萄糖酶 (VviLAC) 基因家族成员在 *Vitis vinifera* L.
- 在葡萄发育过程中探索 *VviLAC* 基因的转录调节网络.
主要方法:
- 在17个染色体中识别了115个*VviLAC*基因家族成员.
- 对基因复制事件的分析,特别是染色体18上的双重复制.
- 在不同果发育阶段对*VviLAC*基因的表达分析.
- 在*VviLAC*表达和关键的二次代谢产物之间进行相关性分析.
- 使用转录组和代谢组数据预测上游的转录监管网络.
主要成果:
- 115个*VviLAC*基因被确定并分为七种类型.
- 在染色体18上的 *VviLAC* 基因中观察到显著的并列重复事件.
- 特定的*VviLAC*基因在水果变色之前和之后表现出不同的表达模式.
- *VviLAC105*表达与复星,复星二元和丁-3-葡萄糖化物水平呈正相关.
- 转录因子 (WRKY,ERF,Dof,MYB) 预测可以调节关键的*VviLAC*基因,如*VviLAC105*和*VviLAC51*.
结论:
- 这项研究提供了葡萄 *VviLAC* 基因家族的全面目录.
- 已识别的*VviLAC*基因及其表达模式为葡萄的发展提供了洞察力.
- 预测的转录调节网络突出了控制*VviLAC*功能和代谢物积累的关键因素.
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