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在患有子宫内膜异位症的患者子宫内膜内有编码和非编码RNA的表达特征
Mi Ran Choi1,2, Hye Jin Chang3, Jeong-Hyeon Heo2
1Laboratory Animal Research Center, Ajou University School of Medicine, Suwon 16499, Republic of Korea.
International journal of molecular sciences
|October 16, 2024
概括
这项研究确定了在不同月经周期阶段在子宫内膜异位症 (EMS) 组织中表达变化的特定长非编码RNA (lncRNA) 和信使RNA (mRNA). 这些发现可能有助于开发新的诊断生物标志物和了解EMS病原体.
科学领域:
- 生殖生物学 生殖生物学
- 分子遗传学 分子遗传学
- 内分泌学 在内分泌学.
背景情况:
- 子宫内膜异位症 (EMS) 是一种复杂的妇科疾病,其特点是子宫外的子宫内膜组织.
- 基本的EMS病原体的分子机制,特别是涉及非编码RNA,仍然不完全理解.
- 在特定的月经周期阶段,子宫内膜的基因表达差异可能为EMS发育提供了洞察力.
研究的目的:
- 在有或没有子宫内膜症的个体的子宫内膜中识别差异表达的长非编码RNA (DElncRNAs) 和信使RNA (DEmRNAs).
- 分析这些表达模式在月经周期的增殖 (P) 和分泌 (S) 阶段.
- 探索已识别的DElncRNAs和DEmRNAs作为诊断生物标记物的潜力,以及它们在EMS病原发生中的作用.
主要方法:
- 总RNA测序是在对照 (CT) 和子宫内膜异位症 (EMS) 患者的子宫内膜组织上进行的.
- 在月经周期的增殖 (P) 和分泌 (S) 阶段收集样本.
- 使用统计分析来识别显著差异表达的lncRNA和mRNA.
主要成果:
- 在增殖阶段,特定的lncRNAs (例如,NONHSAG019742.2,NONHSAT120701.2) 被上调,而其他 (例如,NONHSAG048398.2,NONHSAG016560.2) 与对照组相比,在EMS患者中被下调.
- 在分泌阶段,一些lncRNAs (例如,NONHSAT000959.2,NONHSAT203423.1,NONHSAG053769.2) 的表达增加,而其他 (例如,NONHSAG012105.2,NONHSAG020839.2) 在EMS患者下降.
- 特定的mRNAs (ELP3,NR4A1,LAMB3,HIF1A,PAM) 也在不同阶段在EMS组织中表现出改变的表达模式.
结论:
- 清晰的lncRNA和mRNA表达模式在增殖和分泌阶段与子宫内膜异位症有关.
- 已识别的DElncRNAs和DEmRNAs作为子宫内膜异位症的潜在诊断或预后生物标志物具有前途.
- 这些分子签名为子宫内膜异位症的发病过程提供了宝贵的见解,为未来的研究和治疗策略铺平了道路.
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