甲动物混合血统激酶域样蛋白 (MLKL) 的进化和生物活动
Qingyue Wang1,2,3, Zihao Yuan1,2, Hang Xu1,2
1CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266404, China.
International journal of molecular sciences
|October 16, 2024
概括
混合基因酶域样蛋白 (MLKL) 执行了亡. 这项研究揭示了MLKLL.
科学领域:
- 进化生物学是进化的生物学.
- 细胞生物学 细胞生物学
- 细胞死亡的分子机制
背景情况:
- 混合基因酶域样蛋白 (MLKL) 是哺乳动物编程亡 (亡) 的关键作用因子.
- MLKL的N端域 (NTD) 通过形成等离子体膜毛孔来启动亡,而C端域调节NTD活动.
- 在无脊椎动物中,MLKL的进化保护和生物功能在很大程度上仍未被探索.
研究的目的:
- 调查MLKL在多种多元动物群中的进化分布和功能活动.
- 描述MLKL的遗传关系,特别关注无脊椎动物的骨科.
- 在人类细胞表达时,确定无脊椎动物MLKL N-终端域的尸潜力.
主要方法:
- 在34个甲动物族群中对MLKL存在和保存的生物信息分析.
- 遗传学分析,以构建MLKL的进化分类.
- 功能性试验涉及人类细胞中无脊椎动物MLKL-NTDs的表达,以评估亡诱导.
主要成果:
- 在Deuterostomia和Protostomia (Rotifera) 中检测到MLKL,扩大了其已知的进化范围.
- 罗蒂费拉MLKL表现出明显的进化分歧,与非甲动物MLKL相比,与其他甲动物MLKL相似度更高.
- 来自Rotifera,Echinodermata,Urochordata和Cephalochordata的MLKL-NTDs有效地诱导了人类细胞的亡,突出了保留的功能域.
结论:
- 这项研究为Metazoa中的MLKL古老的进化历史提供了新的见解.
- 它表明无脊椎动物MLKL,特别是来自Rotifera的无脊椎动物,具有功能性尸活动.
- 这些发现强调了MLKL-NTD在广泛的动物物种中执行亡中的保留作用.
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