新的间接抗氧化活性独立于Nrf2,由乳酸细菌发挥
Ayaka Sato1,2, Asami Watanabe1, Kyoji Muraki1
1Department of Molecular and Developmental Biology, Institute of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan.
International journal of molecular sciences
|October 16, 2024
概括
乳酸细菌表现出新的间接抗氧化活性,而不是通过Keap1-Nrf2途径进行介导. 这一发现为探索益生菌开辟了新的途径.
科学领域:
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
- 生物化学 生物化学
背景情况:
- 乳酸细菌 (LAB) 的健康益处众所周知,但它们的抗氧化特性需要进一步研究.
- 氧化应激有助于各种健康问题,使抗氧化剂研究至关重要.
- 斑马鱼幼虫作为研究抗氧化压力因素的抗氧化活性的模型.
研究的目的:
- 用斑马鱼试验研究24种热杀死LAB菌株的抗氧化活性.
- 为了描述LAB中观察到的抗氧化活性机制,特别是H61.1菌株.
- 为了确定抗氧化活性是否涉及Keap1-Nrf2通路.
主要方法:
- 评估LAB菌株在斑马鱼幼虫中减轻过氧化毒性的能力.
- 使用菌株H61.1,研究抗氧化活性的间接性质和时间依赖性.
- 使用Nrf2-Knockout斑马鱼来检查Keap1-Nrf2通路的参与.
- 测试对其他氧化压力因素的有效性,如三甲基氧化物,酸盐和酸盐.
主要成果:
- 所有24种LAB菌株都表现出抗氧化活性,可以抵抗过氧化的毒性.
- 菌株H61表现出最强的间接抗氧化活性,需要超过6小时的预处理.
- 发现H61菌株的抗氧化活性独立于Keap1-Nrf2通路.
- 菌株H61减轻了三丁氧化的毒性,但没有酸或酸的毒性.
结论:
- 已经确定了乳酸细菌的一种新型的间接抗氧化活性.
- 这种活动独立于众所周知的Keap1-Nrf2通路.
- 需要进一步的研究来阐明这种新型LAB抗氧化活性的精确机制和潜在应用.
更多相关视频
10:23Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
Published on: August 23, 2024
699
11:56Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
Published on: May 4, 2018
12.4K
相关概念视频
01:23Microbial Fermentation
1
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1
01:20Fates of Pyruvate
8.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.4K
01:27Microorganisms in Agriculture and Food industry
3
Microorganisms play a crucial role in agriculture and the food industry, contributing to soil fertility, crop protection, and food production. Their functions range from nitrogen fixation and biopesticide production to fermentation and food preservation, making them indispensable to sustainable farming and food safety.Role in AgricultureNitrogen-fixing bacteria, such as Rhizobium (symbiotic) and Azotobacter (free-living), convert atmospheric nitrogen into ammonia through biological nitrogen...
3