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第一个三个月的产前引起的母亲血清蛋白质组的干扰:试点研究

Natalia Starodubtseva^1,2, Alisa Tokareva¹, Alexey Kononikhin¹

¹V.I. Kulakov National Medical Research Center for Obstetrics Gynecology and Perinatology, Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia.

International journal of molecular sciences

|October 16, 2024

概括

这项研究确定了10种母体血清蛋白质作为早期孕前 (PE) 标志物. 使用这些蛋白质的支持矢量机 (SVM) 模型在预测早孕期间的PE风险方面取得了很高的准确性.

科学领域:

产科和妇科产科和妇科
生物化学生物化学
蛋白质组学是指蛋白质组学.

背景情况:

孕前 (PE) 影响高达5%的怀孕,导致显著的孕产妇和新生儿死亡率.
目前的PE风险评估方法存在局限性,需要改进早期检测策略.
早期发现PE对于及时干预和改善母胎结果至关重要.

研究的目的:

调查母亲血清蛋白质组的早期变化,以预测孕前.
为了确定用于早期PE检测的新型血清蛋白生物标志物.
为PE风险分层开发一个准确的预测模型.

主要方法:

在怀孕11-13周使用液体染色学多重反应监测质谱法 (LC-MRM MS) 量化了125个母体血清蛋白.
使用BAK-125套件进行蛋白质量测量.
开发了一个支持向量机 (SVM) 模型,分析了19种母体血清蛋白.

主要成果:

确定了十种潜在的早期PE标志物:APOM,C1QB,LYZ,F2,ALB,AZGP1,TNXB,SERPINA1,ATRN和APOA4. 这些标志物包括:
其中9种蛋白质与PE有先前的关联,证实了它们的可靠性.
SVM模型的AUC为0.91,具有87%的灵敏度和95%的特异性.

结论:

孕产妇血清蛋白质组分析可以识别早期产前的生物标志物.
与常规查相比,基于血清蛋白的SVM模型显示PE的预测能力更强.
这些发现支持蛋白质基因分析对早期PE检测和风险分层的有用性.

关键词:

质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量孕前催产素前症候群是什么怀孕怀孕怀孕怀孕怀孕预后预后预后定量化的蛋白质组学.查检查查检查查检查查检查血液中的血清是血清.

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