Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Inflammatory Response01:28

Inflammatory Response

1.9K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
1.9K
T Cell Types and Functions01:24

T Cell Types and Functions

959
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
959
Inflammatory Response I: Vascular and Cellular01:30

Inflammatory Response I: Vascular and Cellular

11.0K
The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...
11.0K
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

2.5K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.5K
Skin Cancer01:30

Skin Cancer

3.9K
Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...
3.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Self-Applied Daylight Photodynamic Therapy: A Paradigm Shift?

International journal of molecular sciences·2025
Same author

Effect of Combination of Blue and Red Light with Terbinafine on Cell Viability and Reactive Oxygen Species in Human Keratinocytes: Potential Implications for Cutaneous Mycosis.

International journal of molecular sciences·2024
Same author

Determining the Best Noninvasive Test for Peripheral Arterial Disease Diagnosis to Predict Diabetic Foot Ulcer Healing in Patients Following Endovascular Revascularization.

Healthcare (Basel, Switzerland)·2024
Same author

Physical Treatments and Therapies for Androgenetic Alopecia.

Journal of clinical medicine·2024
Same author

Effects of RF Electric Currents on Hair Follicle Growth and Differentiation: A Possible Treatment for Alopecia.

International journal of molecular sciences·2024
Same author

Understanding Active Photoprotection: DNA-Repair Enzymes and Antioxidants.

Life (Basel, Switzerland)·2024

相关实验视频

Updated: Jun 10, 2025

Generation and Culturing of Primary Human Keratinocytes from Adult Skin
10:42

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

16.3K

射频电流调节皮细胞中的炎症过程.

Elena Toledano-Macías1, María Antonia Martínez-Pascual1, Almudena Cecilia-Matilla2

  • 1Photobiology and Bioelectromagnetic Lab, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Hospital Ramón y Cajal, Crta. Colmenar Viejo, km. 9.100, 28034 Madrid, Spain.

International journal of molecular sciences
|October 16, 2024
PubMed
概括

电容电阻转移 (CRET) 疗法增强了角质细胞的增殖,并调节了炎症性细胞因子的释放. 这种物理治疗激活了关键的信号通路,为伤口愈合和炎症控制提供了一种新的方法.

关键词:
欧洲农业基金会 (EGFR) 是一个基金.在ERK1/2中.多元化产业计划 (MMPs) 是指多元化的产业计划.在 NF-κBB 中.细胞因子细胞因子这是一种炎症炎症炎症炎症.质细胞 (keratinocyte) 是一种细胞.物理疗法 物理疗法无线电频率无线电频率的使用.

更多相关视频

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein
08:45

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Published on: September 29, 2021

5.2K
Establishment of a Robust and Reproducible Model of Radiation-Induced Skin and Muscle Fibrosis
07:08

Establishment of a Robust and Reproducible Model of Radiation-Induced Skin and Muscle Fibrosis

Published on: August 31, 2022

1.6K

相关实验视频

Last Updated: Jun 10, 2025

Generation and Culturing of Primary Human Keratinocytes from Adult Skin
10:42

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Published on: December 22, 2017

16.3K
Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein
08:45

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Published on: September 29, 2021

5.2K
Establishment of a Robust and Reproducible Model of Radiation-Induced Skin and Muscle Fibrosis
07:08

Establishment of a Robust and Reproducible Model of Radiation-Induced Skin and Muscle Fibrosis

Published on: August 31, 2022

1.6K

科学领域:

  • 生物医学工程 生物医学工程
  • 皮肤病学 皮肤病学
  • 伤口治愈研究研究 伤口治愈研究

背景情况:

  • 角质细胞对于伤口炎症和再生至关重要,产生调节过程的细胞因子.
  • 以前的研究表明,电容电阻转移 (CRET) 疗法中的射频 (RF) 电流可以影响角质细胞的增殖和细胞因子的产生.
  • 像CRET这样的物理疗法的抗炎作用背后的生物机制需要进一步阐明.

研究的目的:

  • 研究亚热CRET对人类角质细胞 (HaCat) 增殖的影响.
  • 分析CRET对关键细胞因子 (IL-8,MCP-1,RANTES,IL-6,IL-11) 和TNF-α) 的产生的影响.
  • 检查CRET诱导的MMP9,MMP1,NF-κB,ERK1/2和EGFR的表达和激活的变化.

主要方法:

  • 在亚热条件下,人类角质细胞 (HaCat) 暴露于间歇性448kHz的CRET信号.
  • 细胞增殖被评估使用XTT测定.
  • 细胞因子和TNF-α水平通过ELISA量化.
  • 使用免疫光学可视化NF-κB激活.
  • MMP9,MMP1,ERK1 / 2和EGFR的表达和激活通过免疫阻塞测试来确定.

主要成果:

  • 与对照组相比,CRET显著增加了角质细胞的扩散.
  • 观察到MCP-1,TNF-α和IL-6释放的短暂增加,以及IL-8的减少.
  • 治疗CRET导致MMP表达和EGFR,NF-κB和ERK1/2蛋白的激活发生变化.

结论:

  • 克雷特疗法有效调节人类角质细胞的增殖和炎症性细胞因子.
  • 观察到的效应通过EGF受体和ERK1/2/NF-κB信号通路进行介导.
  • 克雷特显示出对调节人类角质细胞的炎症反应的潜力,这与伤口再生有关.