相关实验视频
Updated: Jun 10, 2025

00:07
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
7.2K
了解Dicer1在甲状腺瘤发生中的剂量依赖作用
María Rojo-Pardillo1, Ludivine Godefroid1, Geneviève Dom1
1IRIBHM J. E. Dumont, Université Libre de Bruxelles, 1070 Brussels, Belgium.
International journal of molecular sciences
|October 16, 2024
概括
在甲状腺癌中,Dicer1充当瘤抑制剂. 部分丢失和过度表达Dicer1都会抑制瘤生长,这表明Dicer1是甲状腺癌的潜在治疗标.
科学领域:
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 甲状腺毛囊细胞瘤是常见的内分泌瘤,发病率越来越高.
- 复发影响高达20%的患者,需要研究分子机制.
- 在乳头甲状腺癌 (PTC) 中观察到Dicer1的变化,建议具有足够的瘤抑制活性.
研究的目的:
- 为了研究皮质甲状腺癌 (PTC) 中部分和总的Dicer1损失的体外效应.
- 阐明Dicer1在甲状腺癌细胞增殖,细胞循环,迁移和入侵中的作用.
主要方法:
- 使用CRISPR-Cas9从TPC1.1中生成Dicer1 (+/-) 细胞系.
- 使用siRNA进一步降低Dicer1 (+/-) 细胞中的Dicer1表达.
- 进行了转录基因分析,BrdU染色和transwell测试.
主要成果:
- 部分Dicer1损失 (haploinsufficiency) 和进一步的淘汰导致细胞增殖和迁移的减少.
- 细胞周期分析显示,细胞减速,G0-G1阶段的细胞更多,S阶段的细胞更少.
- 迪塞1过度表达也抑制了扩散和入侵,同时增加了亡.
结论:
- 迪塞1在甲状腺瘤发生过程中起着复杂的作用,作为瘤抑制剂.
- 完全消除和过度表达Dicer1都会抑制甲状腺癌的进展.
- 迪塞1代表了新型甲状腺癌治疗策略的有前途的治疗标.
相关概念视频
Synthesis and Regulation of Thyroid Hormones
4.3K
Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
4.3K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Functions of Thyroid Hormones
2.6K
The thyroid hormone (TH) plays a pivotal role in the intricate orchestration of physiological processes, exerting profound effects on development, metabolism, and homeostasis throughout different life stages.
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
2.6K
Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Inhibition of Cdk Activity
4.7K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.7K

