Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.6K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.6K
Catenins01:23

Catenins

2.3K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.3K
Generation of Straight or Branched Actin Filaments01:14

Generation of Straight or Branched Actin Filaments

2.9K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
2.9K
Cytoskeletal Accessory Proteins01:13

Cytoskeletal Accessory Proteins

3.0K
The cytoskeleton is an essential cell component that plays several structural and functional roles. However, the filaments that make up the cytoskeleton cannot function independently and depend on the accessory or ancillary proteins to effectively carry out their function. Accessory proteins associate with cytoskeletal filaments and their monomers, aiding filament formation and function. They also help in the cross-communication among cytoskeletal filaments. Cytoskeletal accessory proteins are...
3.0K
Actin Filament Depolymerization01:19

Actin Filament Depolymerization

3.1K
Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
In F-actin, the ADF/cofilin proteins...
3.1K
Formation of Higher-order Actin Filaments01:11

Formation of Higher-order Actin Filaments

3.0K
The polymerization of G-actin monomers into filamentous F-actin is a multi-step process. Once the F-actins are formed, they can bundle together in different arrangements to form higher-order networks and regulate cellular functions. Common examples include the formation of lamellipodia and filopodia at the cell's leading edge by actin reorganization in a migrating cell. The microvilli on the brush border epithelial cells are also formed through the F-actin network.
The high-order actin...
3.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Mavacamten shows broad benefit in human and mouse models of MYBPC3-related hypertrophic cardiomyopathy.

Nature cardiovascular research·2026
Same author

GDAP1 orchestrates redox signaling at membrane contact sites to preserve axonal integrity in Charcot-Marie-Tooth disease.

Neurobiology of disease·2026
Same author

Grape Seed Proanthocyanidins Enhance Time-Dependent HO-1 Activation and Improve Redox Homeostasis in Obesity-Induced Hepatic Dysfunction.

Antioxidants (Basel, Switzerland)·2026
Same author

OMA1 protects from liver injury and tumorigenesis during aging by controlling hepatic immunogenicity.

The EMBO journal·2026
Same author

Mitochondria directly interact with the nuclear pore complex.

Nature·2026
Same author

Pro-regenerative fingerprints identified in a sub-population of adult mouse cardiomyocytes by integrative single-cell proteomics.

Genome biology·2026

相关实验视频

Updated: Jun 10, 2025

Labeling F-actin Barbed Ends with Rhodamine-actin in Permeabilized Neuronal Growth Cones
09:14

Labeling F-actin Barbed Ends with Rhodamine-actin in Permeabilized Neuronal Growth Cones

Published on: March 17, 2011

14.8K

ANKK1 是神经F-ACTIN组合的Wnt/PCP支架蛋白.

Laura Domínguez-Berzosa1,2, Lara Cantarero1,2, María Rodríguez-Sanz1

  • 1Laboratory of Neurogenetics and Molecular Medicine, Center for Genomic Sciences in Medicine, Institut de Recerca Sant Joan de Déu, 08950 Barcelona, Spain.

International journal of molecular sciences
|October 16, 2024
PubMed
概括

含有I (ANKK1) 基因的Ankyrin重复和激酶域与突触蛋白相互作用,影响神经发育和F-ACTIN组装. 这项研究表明,ANKK1 TaqIA多态性相关的表型具有大脑结构的基础.

关键词:
ANKK1 的意思是在F-ACTIN的使用中.在FARP1的基础上.塔基亚塔基亚 (Taqia Taqia) 是一个伊斯兰教组织.在Wnt/PCP中使用.吸毒成 吸毒成 是一种成.

更多相关视频

Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons
10:13

Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons

Published on: February 10, 2016

12.0K
Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance
07:53

Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance

Published on: October 21, 2021

3.3K

相关实验视频

Last Updated: Jun 10, 2025

Labeling F-actin Barbed Ends with Rhodamine-actin in Permeabilized Neuronal Growth Cones
09:14

Labeling F-actin Barbed Ends with Rhodamine-actin in Permeabilized Neuronal Growth Cones

Published on: March 17, 2011

14.8K
Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons
10:13

Quantification of Filamentous Actin F-actin Puncta in Rat Cortical Neurons

Published on: February 10, 2016

12.0K
Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance
07:53

Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance

Published on: October 21, 2021

3.3K

科学领域:

  • 神经科学是一个神经科学.
  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学

背景情况:

  • TaqIA多态性与ANKK1 (RIP-激酶) 和DRD2 (多巴胺受体D2) 有关.
  • 人们对ANKK1的功能知之甚少,这阻碍了对TaqIA在精神疾病中的作用的解释.
  • 使用SH-SY5Y神经母细胞瘤模型来研究ANKK1的细胞机制.

研究的目的:

  • 阐明ANKK1在神经元发育中的分子功能.
  • 研究ANKK1与突触蛋白和通路的相互作用.
  • 探索TaqIA多态性相关表型的结构基础.

主要方法:

  • 在SH-SY5Y神经母细胞瘤细胞中研究了ANKK1与FARP1 (一种RhoGEF) 的相互作用.
  • 在富含F-ACTIN的结构中分析了蛋白质的同位分.
  • 研究了ANKK1和FARP1对RhoGTPases和神经分化的影响.
  • 研究了ANKK1与WGEF (另一个Wnt/PCP GEF) 的相互作用.

主要成果:

  • ANKK1与FARP1相互作用,这是RAC1和RhoA的GEF,它们在F-ACTIN结构中同位.
  • ANKK1和FARP1都激活了Wnt/PCP通路;ANKK1促进神经元生成和脊柱外生.
  • Knockdown 的 ANKK1 或 FARP1 影响 RhoGTPase 表达和神经分化.
  • ANKK1调节了WGEF与RhoA的相互作用,在神经元分化过程中改变了相互作用.

结论:

  • 通过F-ACTIN组装,ANKK1在神经元成熟,迁移和脊柱外生方面发挥着至关重要的作用.
  • ANKK1与FARP1和WGEF进行交互,以双向控制F-ACTIN动态.
  • 这些发现为与ANKK1 TaqIA多态性相关的表型提供了潜在的脑结构解释.