不同的Saccharomyces悬浮物与超声波的非侵入性表征
Dominik Geier1, Markus Mailänder1, Iain Whitehead1
1Chair of Brewing and Beverage Technology, TUM School of Life Sciences, Technical University of Munich, 85354 Freising, Germany.
Sensors (Basel, Switzerland)
|October 16, 2024
概括
超声监测提供了一种非侵入性方法,可以在发酵过程中跟踪酵母细胞度和组成. 这种技术准确地反映了酵母的性能,并实现了更好的工艺控制.
科学领域:
- 生物技术是生物技术.
- 工艺工程的过程工程.
- 分析化学 分析化学
背景情况:
- 监测酵母细胞数量和基质度对于优化发酵过程至关重要.
- 目前用于监测生物悬浮物的方法往往是侵入性的或因检测问题而受到限制.
- 为了高效的工业发酵,需要进行非侵入性实时监测.
研究的目的:
- 调查超声波测量的适用性,以表征酵母悬浮.
- 评估超声波监测酵母细胞 (分散阶段) 和发酵介质 (连续阶段) 的能力.
- 为了比较超声波的性能与三种不同的Saccharomyces酵母菌株.
主要方法:
- 利用超声波信号来分析酵母悬浮.
- 测量了超声波衰减系数和声音的速度.
- 相关的超声波参数与不同的酵母度 (0.0-1.0 wt%) 和细胞计数.
- 研究了细胞直径和体积密度对超声波衰减的影响.
主要成果:
- 对所有测试的酵母,超声衰减和声音速度与酵母度和细胞数量 (R2 > 0.95) 呈现线性增加.
- 超声波信号衰减受酵母细胞直径和体积密度的影响.
- 声音速度的变化部分归因于液体介质组成的变化.
结论:
- 超声波技术对于监测工业发酵中的酵母细胞度和特征是有效的.
- 这些发现支持开发利用超声波对发酵过程进行有针对性的控制策略.
- 超声波为传统监测方法提供了一个非侵入性,实时的替代方案.
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