通过下一代测序识别黑 (Nigella sativa) 微RNA及其对二次代谢物生物合成的影响

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Updated: Jun 10, 2025

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
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RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

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Andrea G Uriostegui-Pena1, Almendra Reyes-Calderón1, Claudia Gutiérrez-García1

  • 1NatProLab, School of Engineering and Sciences, Tecnologico de Monterrey, Queretaro 76130, Mexico.

Plants (Basel, Switzerland)
|October 16, 2024
PubMed
概括

这项研究揭示了microRNAs (miRNAs) 调节Nigella sativa的二次新陈代谢. 研究人员确定了针对关键酶的新型和保存的miRNA,为增强药物化合物生产铺平了道路.

关键词:
黑子/黑子种子高通量测序的高通量测序药用植物 药用植物微RNA (miRNA) 是一种微型RNA.二次新陈代谢二次新陈代谢

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MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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