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Updated: Jun 10, 2025

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In vitro Organoid Culture of Primary Mouse Colon Tumors
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无机聚酸盐通过TRPM8受体信号通路促进结肠直肠癌的生长
Valentina Arrè1, Francesco Balestra1, Rosanna Scialpi1
1Personalized Medicine Laboratory, National Institute of Gastroenterology "S. de Bellis", IRCCS Research Hospital, Via Turi 27, Castellana Grotte, 70013 Bari, Italy.
Cancers
|October 16, 2024
概括
无机聚酸盐 (iPolyP) 在结直肠癌 (CRC) 中升高,并通过TRPM8受体发出信号来驱动瘤生长,促进细胞增殖.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 大肠直肠癌 (CRC) 呈现出一种支持炎症的微环境,支持瘤生长.
- 无机聚酸盐 (iPolyP) 是一种高能量聚合物,调节哺乳动物细胞功能,包括增殖.
- 有证据表明,iPolyP参与致癌的情况需要进一步调查.
研究的目的:
- 研究无机聚酸盐 (iPolyP) 在结直肠癌 (CRC) 扩散中的作用.
- 使用体外和体外模型评估iPolyP对瘤生长的影响.
主要方法:
- 对人类CRC组织中的iPolyP水平与周围瘤组织的分析.
- 使用CRC细胞系 (Caco-2,SW620) 进行TRPM8抑制和RNA干扰的体外研究.
- 使用CRC衍生器官的ex vivo研究.
- 细胞周期进展和基因表达的评估 (ccnb1).
主要成果:
- 与相邻的正常组织相比,CRC瘤组织中的iPolyP水平显著增加.
- iPolyP通过TRPM8受体促进CRC细胞增殖,TRPM8受体对于这种效果至关重要.
- iPolyP 增强了ccnb1的表达,使癌细胞进入线粒分裂.
- 用iPolyP治疗的CRC有机体显示尺寸增加.
结论:
- iPolyP在结直肠癌中升高,并支持瘤细胞的增殖.
- iPolyP通过TRPM8离子通道起作用,增强细胞周期的进展.
- iPolyP代表了一种潜在的新的结直肠癌扩散早期生物标志物.
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