相关实验视频
Updated: Jun 10, 2025

08:26
Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
Published on: June 25, 2018
6.6K
菌的N-甘氨酸的多样性 食品补充剂 挑战 目前的物种分类
Réka Mócsai1, Johannes Helm1, Karin Polacsek1
1Department of Chemistry, BOKU University, Muthgasse 18, 1180 Vienna, Austria.
Foods (Basel, Switzerland)
|October 16, 2024
概括
微藻N-甘氨酸的多样性挑战了商业产品中的物种识别. 分析N-甘氨酸揭示了不一致性,表明它们用于分类学分类和确保产品真实性的使用.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
背景情况:
- 越来越多的N-甘氨酸因其在Chlorella菌株和产品中的可变性而得到认可.
- 以前的分类学分类可能没有完全捕捉到Chlorella物种内的N-glycan多样性.
研究的目的:
- 重新审视基于N-甘氨基的商业微藻产品的物种名称和产品真实性.
- 评估菌产品中N-甘氨酸的多样性及其对分类学的影响.
主要方法:
- 使用矩阵辅助飞行时间质谱法 (MALDI-TOF MS) 来分析172种商业微藻产品中的N-甘氨酸.
- 用质谱检测对多孔石墨碳进行染色学,以支持N-glycan分析.
主要成果:
- 标记为C. vulgaris的产品中,只有9%的产品表现出典型的N-甘氨酸模式,而C. sorokiniana菌株发现了显著的差异.
- 很大一部分产品 (41%) 显示出常见的N-甘氨酸类型,而26%显示出独特或罕见的模式,挑战了所述的物种名称.
结论:
- 在商业微藻产品中,N-glycan签名提供了一个强大的工具,可以挑战和改进商业微藻产品中的当前物种名称.
- 该研究强调了N-甘氨酸在定义产品身份和改善微藻的分类学分类方面的潜力.
相关概念视频
Oligosaccharide Assembly
2.8K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
2.8K
Diversity of Protists I
1
Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...
1
Protein Glycosylation
6.8K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Glycosylation occurs in...
6.8K
Green Algae
Green algae, also referred to as chlorophytes, are different from red algae in having the chloroplasts containing chlorophylls a and b, which give them their distinct green hue. However, they lack phycobiliproteins, preventing them from developing the red or blue-green pigmentation seen in red algae. In terms of photosynthetic pigment composition, green algae closely resemble plants and share a close evolutionary relationship with them. Taxonomically Green algae belong to Phylum Chlorophyta in...
Evolutionary Relationships through Genome Comparisons
5.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.7K

