由于干扰而导致登革热双组测定负控制线的一个奇怪案例 - 一个案例报告
Sheila X Soh1, Tze Ping Loh1, Sharon Saw1
1Department of Laboratory Medicine, National University Hospital, Singapore.
Annals of clinical biochemistry
|October 16, 2024
概括
一种高分子量物质干扰了登革热非结构蛋白1 (NS1) 测试,导致没有对照线. 当这种干扰发生时,可能需要替代登革热诊断套件.
科学领域:
- 临床诊断 临床诊断 临床诊断
- 免疫染色学是一种免疫染色学.
- 生物化学 生物化学
背景情况:
- 登革热非结构蛋白1 (NS1) 检测对于早期登革热诊断至关重要.
- 免疫染色学测试 (ICAs) 被广泛用于登革热NS1检测.
- 测试控制线对于验证测试结果至关重要.
研究的目的:
- 为了调查登革热NS1免疫染色学试验中缺少对照线的原因.
- 为了确定干扰物质的性质.
- 评估实验室程序对测定性能的影响.
主要方法:
- 用多个登革热NS1ICA测试患者血清.
- 使用的干扰减轻技术:稀释,聚乙烯糖醇 (PEG) 沉和超过.
- 用于验证实验程序的控制血清.
主要成果:
- 在两个不同的登革热NS1 ICAs上缺席的对照线表明了测试干扰.
- 一种高分子量 (>100 kDa) 的物质被涉及.
- 稀释部分恢复了控制线;PEG沉和过影响了测试性能和生物标志物的完整性.
结论:
- 一种高分子量干扰剂可以破坏登革热NS1 ICA控制线,可能通过影响IgY-anti-IgY结合.
- 实验室程序如PEG沉和过可以改变生物标志物水平,应谨慎使用并进行适当的控制.
- 为了控制这种干扰,可能需要使用不同的抗体-抗原系统的替代登革热诊断套件.
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