进化起源和创新塑造了哺乳动物PRPS酶复合物的演化起源和创新
Bibek R Karki1, Austin C Macmillan1, Sara Vicente-Muñoz2
1Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
bioRxiv : the preprint server for biology
|October 16, 2024
概括
基酸盐合成酶 (PRPS) 在真核生物中从单个基因演变为复杂的多重分子. 这个复合体调节细胞生物化学,影响新陈代谢和繁殖.
科学领域:
- 生物化学 生物化学
- 进化生物学 进化生物学
- 分子生物学分子生物学
背景情况:
- 基酸合成酶 (PRPS) 酶对于连接中央碳代谢和核酸合成至关重要.
- 多个PRPS编码基因是真核生物体的特征.
研究的目的:
- 为了追踪哺乳动物PRPS同类的进化起源.
- 定义五种哺乳动物PRPS同类物 (三种异酶和两种相关蛋白质) 的个别功能.
- 为了阐明PRPS多重复合物的组装和调节.
主要方法:
- 对具有不同PRPS亚单元组成的同源纤维细胞克隆的分析.
- 在PRPS复合体内的子单元相互作用的调查.
- 评估PRPS组件改变的细胞中的代谢流量和增殖能力.
主要成果:
- 哺乳动物PRPS存在于一个大型,异质的多重体复合体中,具有明显的子单元相互作用.
- 失去特定的PRPS子单元 (PRPS2,PRPSAP1,PRPSAP2) 会导致异常的PRPS1同类寡合体,降低代谢流量,并影响增殖.
- 在重复的PRPS基因中的进化创新创造了专门的角色,和翻译控制微调复杂的活动.
结论:
- 进化将单一的PRPS基因转化为一个复杂的多重复合体,具有专门的功能.
- PRPS复合体在调节细胞生物化学,代谢灵活性和组织专业化方面发挥着至关重要的作用.
- 了解PRPS复杂的动态,可以深入了解高级生命形式的代谢调节.
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