对微生物转化潜力的基因组和转录组分析,以及瑞士湖泊沉积物中的基因表达
Kathrin B L Baumann1, Alessandra Mazzoli2, Guillem Salazar3
1Eawag, Swiss Federal Institute of Aquatic Science and Technology, 6047 Kastanienbaum, Switzerland.
ISME communications
|October 16, 2024
概括
人类活动改变了循环,影响淡水沉积物. 微生物的去除,主要是通过化和脱化,受湖泊类型和有机物质的影响,而不仅仅是地质化学.
科学领域:
- 环境微生物学环境微生物学
- 生物地质化学生物地质化学
- 水生生态学 水生生态学
背景情况:
- 全球 (N) 循环因人类活动而改变,在水生生态系统中增加了N.
- 淡水沉积物是固定N循环和去除的关键地点.
- 控制沉积物中的微生物N去除途径的环境因素尚未完全理解.
研究的目的:
- 在湖泊沉积物中研究微生物N转化基因的丰富性和表达.
- 确定地酸盐去除的环境驱动因素.
- 了解对微生物N循环社区及其活动的控制.
主要方法:
- 对12个瑞士湖泊沉积物的元基因组学和元转录组学分析.
- 在不同沉积率和热量状态的湖泊中进行比较.
- 基因数据,地球化学和有机物质特征之间的相关性分析.
主要成果:
- 化与脱化相结合,是主要的微生物N损失途径.
- 湖泊类型 (受农业影响,原始的阿尔卑斯山,大/深) 显示出不同的N转化基因配置文件.
- 沉积有机物特性与微生物N循环社区结构有很强的相关性.
- 基因表达偏离了丰富性,与地质化学没有相关性;暗示了神秘的N循环.
- 大而深的湖泊表现出较低的N基因表达,可能是由于长时间的有机物矿化.
结论:
- 岩损失主要是由化-脱结合驱动的,受湖泊特征和有机物质的影响.
- 微生物群落结构与有机物组成密切相关.
- 基因表达模式表明复杂的调节和潜在的神秘N循环.
- 水柱有机物处理可以抑制深湖中的盆地N去除基因表达.
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