相关实验视频

Updated: Jun 10, 2025

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

表达HPV16/18 E7的重组腺病毒在C33A细胞中调高HDAC6和DNMT3B基因

Yunting Shao¹, Pir Tariq Shah¹, Qisheng Su¹

¹Faculty of Medicine, School of Basic Medical Sciences, Dalian University of Technology, Dalian, China.

Frontiers in cellular and infection microbiology

|October 16, 2024

概括

高风险的人类乳头瘤病毒 (HPV) E6和E7蛋白质通过上调HDAC6和DNMT3B来驱动子宫癌. 这些基因在癌症途径中至关重要,为HPV相关癌症提供潜在的治疗点.

科学领域:

在瘤学瘤学.
病毒学病毒学
分子生物学分子生物学

背景情况:

高风险的人类乳头瘤病毒 (HPV) 与宫,门和口腔癌有关.
HPV E6 和 E7 瘤蛋白是细胞转化和癌症发展的关键驱动因素.
需要进一步调查HPV E6/E7对宿主蛋白质的全部影响.

研究的目的:

研究HPV16/18 E6和E7瘤原蛋白对宿主细胞蛋白质的全球调控作用.
通过HPV E6/E7表达,在宫癌细胞中识别差异表达基因 (DEGs).
探索DEG与关键癌症相关信号通路的相关性.

主要方法:

构建表达HPV16/18 E6,E7或两者的复合腺病毒.
感染C33A宫癌细胞以持续的E6/E7表达.
RNA测序 (RNA-Seq) 用于转录组分析和DEGs的识别.
定量逆转录PCR (qRT-PCR) 和西布洛特用于基因表达验证.

主要成果:

过度表达的HPV16/18 E6/E7上调的GP6,CD36,HDAC6,ESPL1和DNMT3B. 这种过度表达.
HDAC6和DNMT3B与DNA复制,增殖,G2M检查点,p53和PI3K/AKT/mTOR通路有显著的相关性.
证实HPV16/18 E7可上调HDAC6和DNMT3B的表达.

结论:

再组合腺病毒成功表达了HPV16/18 E6/E7,保留了感染力.
在HPV16/18 E7上调HDAC6和DNMT3B,影响细胞周期和癌症途径.
HDAC6和DNMT3B是HPV相关癌症的潜在治疗点,需要进一步研究E6/E7的瘤机制.

关键词:

在DNMT3B中,我们可以使用DNMT3B.E6 / E7 基因基因 E6/E7 基因在 HDAC6 中.高风险的HPV感染者再组合病毒的复合病毒.

更多相关视频

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

相关概念视频

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...