Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Micro-comb 3D printing: rapid fabrication of tissue-guiding substrates using micro-embossed nozzles.

Biofabrication·2026
Same author

Single-Molecule Imaging and Spectroscopy Enables Quantification of Location-Dependent Light-Matter Interactions on Nanoantennas.

Small science·2026
Same author

Liposome purification from micromolar protein background using diffusiophoretic trapping.

Nanoscale·2026
Same author

Single-shot Stokes polarimetry of plasmon-coupled single-molecule fluorescence.

Nanophotonics (Berlin, Germany)·2025
Same author

Hot Charge Carriers Mediate Regioselective Thiol Cleavage and Biofunctionalization of Gold Nanoparticles.

ACS applied materials & interfaces·2025
Same author

In-Solution Characterization of Biomolecular Interaction Kinetics under Native Conditions.

Analytical chemistry·2025
Same journal

Formation of Bimetallic Nanoparticles via Exsolution Using a Reducible Metal Oxide Capping Layer.

ACS nano·2026
Same journal

Cold-Driven Thermoelectric Patch for Postoperative Tumor Control.

ACS nano·2026
Same journal

Chemically Fueled Interfacial Supramolecular Polymerization.

ACS nano·2026
Same journal

Tactile Neuromorphic Ion-Gated Vertical Transistor Displays Enabling Dual-Output Reservoir Computing.

ACS nano·2026
Same journal

In Situ Oxygen Shuttling within a Bilayer Electrified Membrane Enables Aeration-Free Electro-Fenton Water Purification.

ACS nano·2026
Same journal

Single Atoms as Growth Directors: From Graphene Edges to Atomically Precise Interfaces in 2D Materials.

ACS nano·2026
查看所有相关文章

相关实验视频

Updated: Jun 10, 2025

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.8K

点分布函数变形解锁球形纳米粒子上的3D定位显微镜.

Teun A P M Huijben1, Sarojini Mahajan2, Masih Fahim1

  • 1Department of Health Technology, Technical University of Denmark (DTU), Kongens Lyngby 2800, Denmark.

ACS nano
|October 16, 2024
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新模型,使用超分辨率显微镜准确地绘制纳米粒子 (NP) 表面功能. 这种快速而精确的方法揭示了NP表面覆盖率,这对于药物输送和生物传感等应用至关重要.

关键词:
在DNA涂料上.纳米颗粒是一种纳米粒子.塑制剂的使用方法一个点差函数的点差函数.单分子局部化显微镜.表面功能化的功能化.

更多相关视频

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.4K
Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

12.8K

相关实验视频

Last Updated: Jun 10, 2025

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.8K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.4K
Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

12.8K

科学领域:

  • 纳米技术 纳米技术
  • 生物物理学的生物物理.
  • 显微镜的使用方法

背景情况:

  • 纳米粒子 (NP) 在生物传感,药物输送和光热疗法中至关重要.
  • 表面功能化极大地影响了NP的性能.
  • 传统的超高分辨率显微镜在光体局部化中与NP诱导的工件作斗争.

研究的目的:

  • 开发一个精确的分析点传播函数 (PSF) 模型,用于球形NP附近的光体.
  • 在超分辨率成像中克服NP引起的系统错位.
  • 为了能够精确地在NP上3D定位表面功能组.

主要方法:

  • 导出了一个球形NP附近的光体的分析PSF模型.
  • 与数值方法相比,实现了四个数量级的加速度.
  • 将模型应用于DNA-PAINT超分辨率显微镜数据的DNA涂层黄金NP.

主要成果:

  • 在提取表面函数组的3D位置时证明<5nm的精度.
  • 在NP上发现不均的表面覆盖.
  • 验证了模型的准确性和速度用于现实世界的应用.

结论:

  • 开发的分析PSF模型在超分辨率显微镜中准确地纠正NP诱导的工件.
  • 该方法提供了NP表面功能化的快速,精确和可访问的分析.
  • 这种方法有望成为纳米医学和生物传感中成像NP的标准.