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使用修改的水友互动液体染色学量化新型甲福明乳液的皮肤透率

Derek J Maloney¹, Jianying Zhang¹, Satyaj Bhargava¹

¹MechanoBiology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA.

|October 16, 2024

概括

这项研究量化了通过猪耳皮的甲福明乳液透率,使用水友相互作用液态色谱 (HILIC). HILIC 方法准确地测量了甲的透率,显示它随着时间的推移而增加.

科学领域:

药理学药理学是指药理学的学科.
分析化学分析化学
皮肤病学皮肤病学

背景情况:

通过皮肤递送药物系统需要方法来量化药物透.
在透性研究中,猪耳皮作为人类皮肤的可行模型.
甲胺 (Met) 是一种广泛使用的抗糖尿病药物,具有局部应用的潜力.

研究的目的:

测量甲福明乳液通过猪耳皮的透率.
验证使用水友互动液体染色学 (HILIC) 来分离甲胺与皮肤污染物的使用.
建立一种可靠的方法来测量皮肤病学研究中的甲福明透率.

主要方法:

使用弗朗茨细胞透试验来评估6%的甲福林乳液.
使用HILIC的高性能液态色谱 (HPLC) 用于样品分析.
甲胺的量化基于其9分钟的保留时间 (RT),使用酸性,极性移动相和正常相柱.

主要成果:

在6%的乳液中发现了9分钟RT的明确的甲福林峰值,而在0%的对照中则没有.
甲福林峰值明显与生物污染物分开,在2-3分钟RT之间排泄.
在24小时内,美特福林的透率线性增加,达到应用剂量的36.8%.

结论:

水友交互液体色谱 (HILIC) 能够有效地将甲福明与皮肤脂质和蛋白质分离.
这种经过验证的HILIC方法允许准确量化通过猪耳皮的甲福林透.
这些发现支持使用HILIC来分析局部药物输送和透研究.

关键词:

螺旋体螺旋体螺旋体在HPLC中使用HPLC.弗朗茨细胞检测试验甲胺是一种甲胺.猪耳皮肤模型模型保留时间保留时间通过皮肤透.通过皮肤传递的传递.

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