EIF4A3诱导的循环RNACircDdb1通过编码新型蛋白质CircDdb1-867aaaa促进肌肉缩
Xiaolan Zhu1,2, Tingting Yang1,2, Yongjun Zheng3
1Cardiac Regeneration and Ageing Lab, Institute of Geriatrics (Shanghai University), Affiliated Nantong Hospital of Shanghai University (The Sixth People's Hospital of Nantong) and School of Life Sciences, Shanghai University, Nantong, 226011, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|October 16, 2024
概括
循环RNAs (circRNAs) 在肌肉缩中起作用. 一个特定的circRNA,circDdb1,通过抑制蛋白质翻译促进肌肉消耗,是潜在的治疗标.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 生理学 生理学 生理学
背景情况:
- 骨肌肉缩是一种使人衰弱的疾病,对潜在的分子机制的理解有限.
- 循环RNAs (circRNAs) 正在成为各种生物过程中的关键调节者,但它们在肌肉缩中的作用仍然在很大程度上未被探索.
研究的目的:
- 为了研究circRNAs在骨肌肉缩中的参与.
- 确定调节肌肉缩的特定circRNA,并阐明它们的作用机制.
主要方法:
- 在各种肌肉缩模型 (体内和体外) 中分析circRNAs的表达.
- 已识别的circRNAs的功能获取和功能丧失研究.
- 蛋白质与蛋白质相互作用和下游信号通路的识别.
主要成果:
- 由DDB1基因衍生而来的一个保存的circRNA,circDdb1,在包括衰老在内的多种肌肉缩条件下显著上调.
- 宫外表达 circDdb1 诱导肌肉缩,而其抑制改善由各种刺激 (德克萨米他,TNF-α,Ang II,缩,不运动) 引起的缩.
- circDdb1编码了一种新型蛋白质 (circDdb1-867aa),该蛋白质增强了eEF2酸化,减少了蛋白质转化并促进了缩.
结论:
- circDdb1在多种不同的肌肉缩病理中起到共同的分子调节者的作用.
- circDdb1代表了对抗骨肌肉消耗的有希望的治疗标.
相关概念视频
01:13Formation of Muscle Fibers from Myoblasts
4.8K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
4.8K
02:23RNA Editing
8.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
8.9K
02:27Nonsense-mediated mRNA Decay
10.6K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.6K
01:31Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
02:55The Nucleolus
8.7K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.7K
02:53Ribosomal RNA Synthesis
13.1K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.1K