相关实验视频

Updated: May 7, 2026

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Published on: December 17, 2015

对 Porphyromonas gingivalis Lipopolysaccharide 诱导的人类牙角质细胞早期基因表达的转录组分析

Mahyar Ostadkarampour¹, Edward E Putnins¹

¹Department of Oral Biological and Medical Sciences, Faculty of Dentistry, The University of British Columbia, Vancouver, British Columbia, Canada.

Journal of periodontal research

|October 16, 2024

概括

葡萄牙牙的脂聚糖 (PgLPS) 触发了牙细胞的早期免疫反应. PgLPS对IL36G进行上调,可能在牙周病的发病过程中起到警示作用.

科学领域:

口腔微生物学口腔微生物学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

Porphyromonas gingivalis 脂多糖 (PgLPS) 是一个关键的毒性因素.
PgLPS在牙上皮细胞中驱动先天性免疫反应,导致炎症.

研究的目的:

为了研究由PgLPS引起的人类牙角质细胞 (HGK) 的早期基因表达变化.
阐明参与PgLPS初始反应的分子途径.

主要方法:

用PgLPS治疗的HGK细胞的转录组分析 (RNAseq).
基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.
使用RT-qPCR验证关键基因表达.

主要成果:

确定了25个差异表达基因 (DEGs) 和两个丰富的通路.
PgLPS诱导了与尿素酶/凝血相关的基因 (PLAU,PLAUR,SerpinB2),与增加的细胞迁移相关.
在IL-1受体结合和细胞因子通路 (IL36G,IL1B,IL1RN,CXCL14) 中,PGLPS上调基因.

结论:

PgLPS对PLAU,PLAUR和SerpinB2的升级与增强的细胞迁移有关.
PgLPS显著诱导IL36G和IL1B,IL36G与其对手IL36RN相比表达增加.
早期的IL36G上调可能会起到警示作用,在牙周病期间启动HGK的先天免疫反应.

关键词:

一个IL-1家族.基因和基因组的京都百科全书 (KEGG)P. gingivalis 是一种脂多糖类糖类.对RNA序列分析进行分析.补充级联级联的补充.基因存在学 (GO)牙周病是一种牙周病.

更多相关视频

Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632

Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632

Published on: November 6, 2021

Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro

Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro

Published on: March 28, 2025

相关概念视频

Formation of Lipopolysaccharides

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...