在压力下通过MAPK介导的Cdc20Slp1减弱APC/C激活的负调节
Li Sun1, Xuejin Chen1, Chunlin Song1
1State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, China.
eLife
|October 16, 2024
概括
线素激活蛋白激酶 (MAPKs) 通过调节裂变酵母中的APC/C活性来抑制细胞分裂. 这种依赖MAPK的路径对于在压力下保持螺旋组装检查点 (SAC) 至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 线粒体酶的开始是细胞循环的关键阶段,由激酶调节.
- 甲基因激活蛋白激酶 (MAPK) 参与了这一过程,但确切的机制和进化保护尚不清楚.
- 裂变酵母 (Schizosaccharomyces pombe) 作为研究保存的真核细胞过程的模型.
研究的目的:
- 为了研究MAPKs在调节Schizosaccharomyces pombe的亚纳相发作中的作用.
- 阐明MAPKs控制亚纳酶促进复合体/环体 (APC/C) 的分子机制.
- 为了确定MAPK依赖的线粒细胞调节是否被保留并在压力条件下发挥作用.
主要方法:
- 使用了Schizosaccharomyces pombe作为一个模型生物体.
- 研究了Slp1Cdc20通过MAPKs的相互作用和酸化,特别是Pmk1.
- 评估了Pmk1酸化对Slp1Cdc20无处不在和降解的影响.
- 检查了Pmk1在环境压力下维持组装检查点 (SAC) 的作用.
主要成果:
- 在S. pombe中,两个MAPK合作,在SAC活跃时抑制APC/C活性.
- 该MAPK Pmk1酸化Slp1Cdc20,APC/C联合激活剂,促进其降解.
- 通过pmk1介导的酸化对于在环境压力下维持SAC功能至关重要.
- 这与Cdk1无法通过酸化促进Slp1Cdc20降解形成鲜明对比.
结论:
- 建立了一个新的机制,其中MAPKs在应对压力刺激时负面调节APC/C活动.
- 证明了在真核生物中依赖MAPK调节相进入的进化性保护.
- 通过MAPK信号传递,为了解压力如何影响细胞周期进展提供了一个新的框架.
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