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线粒体DNAG-四重复的可视化与Isaindigotone衍生近红外化探针

Yuan Zhang¹, Yidan Cheng¹, Xianjun Liu²

¹State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, P. R. China.

Analytical chemistry

|October 16, 2024

概括

研究人员开发了一种新的近红外光探头ISAP,用于成像线粒体DNAG四重复 (mtDNAG4s). 这种先进的探测器提高了灵敏度,并允许在活细胞和体内可视化mtDNA G4动态.

科学领域:

分子生物学分子生物学
生物物理学的生物物理.
细胞生物学细胞生物学

背景情况:

线粒体DNA的G-四复合体 (mtDNA G4s) 参与调节线粒体功能.
目前对mtDNA G4s的光探测器缺乏灵敏度,并且在更短的波长中工作.
需要先进的探测器来研究生物系统中的mtDNA G4动态.

研究的目的:

开发一种新型,灵敏的近红外 (NIR) 光探针,用于成像mtDNA G4s.
调查探测器在活细胞和体内可视化mtDNA G4s的能力.
为了探索mtDNA G4s在各种细胞压力下的生物作用.

主要方法:

用不同的电子捐赠群组合成来自伊桑迪戈的光探针.
探测器性能的表征,包括吸收,发射和光激活.
分子对接模拟以预测与G4结构的结合相互作用.
在活细胞中进行细胞成像研究,以评估线粒体向和特异性.
在携带瘤的小鼠模型中的体内成像.

主要成果:

开发的探测器ISAP在对G4s的反应中表现出NIR吸收/发射和高光激活.
ISAP展示了优秀的线粒体向和活细胞中mtDNA G4s的特定成像.
探测器成功地可视化了在糖解和氧化应激下mtDNA G4的动态.
在瘤模型中,ISAP显示出对mtDNA G4s体内成像的能力.

结论:

ISAP是一种敏感和特定的NIR光探头,用于成像mtDNA G4s.
这种探测器可以在活细胞和体内研究mtDNA G4动态.
ISAP作为一个有价值的工具,用于调查mtDNA G4s.的生物学作用.

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