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通过测序进行基因定型;用于识别和绘制新开发的小麦突变系中诱导突变的策略

Sana Zulfiqar¹, Mehboob-Ur- Rahman², Sayyad Ali Raza Bukhari³

¹Plant Genomics and Molecular Breeding Laboratory, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Punjab, 38000, Pakistan.

Functional & integrative genomics

|October 16, 2024

概括

物理变异原体创造了具有多样化遗传资源的小麦新型突变. 高吞吐量测试确定了许多存在/缺席变异 (PAV) 标志物,揭示了与应激耐受性和突变物中改善的农学特征相关的PAV.

科学领域:

植物遗传学植物遗传学
分子生物学分子生物学
农业学是一种农业学.

背景情况:

物理变异原体可以诱导植物的遗传变异,为作物改进创造新的资源.
了解小麦突变种的遗传多样性对于开发弹性品种至关重要.

研究的目的:

使用高吞吐量GBS-DArTseqTM.估计33种稳定小麦突变的遗传多样性.
识别和描述小麦突变中的新型遗传变异,特别是存在/缺失变异 (PAVs).

主要方法:

高吞吐量GBS-DArTseqTM试验被用于分析33种小麦突变种和野生种类.
存在/缺席变异 (PAV) 标记被确定并分布在小麦染色体中.
使用小麦转录组,COG和GO-terms数据库进行了PAVs的功能性表征.

主要成果:

总共有1,57,608个PAV标记物被确定,其中B和D亚基因组的度更高.
3,252个PAV在突变中独一无二地存在,在野生类型中不存在.
PAV与参与信号传递,新陈代谢,防御,耐病性,非生物性压力,光合作用效率,谷粒大小和产量的途径有关.

结论:

小麦突变体表现出显著的遗传多样性,其特点是独特的PAVs.
这些PAV具有提高小麦育种中耐压力和关键农学特征的潜力.
这项研究为分子遗传学家和育种者提供了宝贵的见解,以利用诱导的遗传多样性开发弹性小麦品种.

关键词:

功能性注释功能性注释遗传多样性遗传多样性突变的基因系是突变的基因系下一个基因测序.存在/缺席变化存在/缺席的变化.

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相关概念视频

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.