感染土壤化剂的新型病毒家族的活动与宿主区分的同时发生
Sungeun Lee1, Christina Hazard1, Graeme W Nicol1
1Univ Lyon, CNRS, INSA Lyon, Université Claude Bernard Lyon 1, Ecole Centrale de Lyon, Ampère, UMR5005, Ecully 69134, France.
The ISME journal
|October 16, 2024
概括
土壤病毒与氨氧化细菌 (AOB),氨氧化古生物 (AOA) 和酸盐氧化细菌 (NOB) 积极相互作用,影响循环. 这项研究描述了与这些关键微生物群体相关的新型病毒.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 病毒学 病毒学
背景情况:
- 化学和自转性化剂 (AOA,AOB,comammox,NOB) 对于循环至关重要.
- 土壤病毒群体是动态的,但它们的功能作用,特别是化剂相互作用的功能作用,尚不清楚.
- 由于病毒多样性和宿主链接困难,感染低丰度化剂的病毒的特征具有挑战性.
研究的目的:
- 调查化剂生长和活跃的土壤病毒种群之间的关联.
- 为了确定以过程为重点的分析是否可以从土壤中获得高质量的病毒基因组.
- 描述感染氨氧化细菌 (AOB),氨氧化古生物 (AOA) 和酸盐氧化细菌 (NOB) 的新型病毒.
主要方法:
- 使用差异性宿主抑制来丰富特定的化剂组及其病毒的目标化现场化研究.
- 监测化剂的生长,并将其与相关病毒的丰富度相关联.
- 丰富的dsDNA病毒的基因组分析,以描述它们的基因组和功能.
主要成果:
- 化剂的生长与各自病毒的丰富性相关,证实了活跃的宿主病毒相互作用.
- 发现了61个高质量/完整的dsDNA病毒基因组 (35-173 kb),与已知病毒家族的相似性很小.
- AOA病毒缺乏amoC基因,但有些人编码MCO1,这表明AOA在铜获取和能量代谢中的作用.
结论:
- 聚焦的化研究对于在土壤化剂社区中表征活跃宿主病毒相互作用是有效的.
- 感染AOA,AOB和NOB的土壤病毒是动态的,在循环中起着重要作用.
- 确定了与土壤化剂相关的新型病毒,扩大了我们对陆地生态系统中的病毒多样性和功能的理解.
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