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基于的氧化物对内皮细胞动力学和细胞外矩阵重塑的影响

Beatriz de Almeida Camargo¹, Geórgia da Silva Feltran¹, Célio Junior da Costa Fernandes¹

¹Department of Chemical and Biological Science, Institute of Biosciences, UNESP - São Paulo State University, Botucatu, São Paulo 18618-970, Brazil.

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)

|October 16, 2024

概括

稳定 (ZrO2) 增强了内皮细胞的增殖,并影响了细胞外基质的重塑,这对于牙植入物骨质整合至关重要. 这种材料显示出在牙科应用中改善血管和组织整合的潜力.

科学领域:

生物材料科学生物材料科学
细胞生物学细胞生物学
牙科植入物学牙科植入物学

背景情况:

(ZrO2) 在牙科修复中受到青,因为它的美学和生物相容性.
内皮细胞功能和细胞外基质 (ECM) 重塑对于骨质整合至关重要.

研究的目的:

为了研究稳定 (ZrO2) 对内皮细胞行为的影响.
在牙科植入物环境中分析受ZrO2影响的ECM重塑过程.

主要方法:

在静态和剪切应力下在ZrO2丰富介质中培养的人类脉内皮细胞 (HUVECs).
使用SEM,EDX和XRD进行ZrO2的表面表征.
评估基因和蛋白质表达 (RT-qPCR,西式涂抹) 和矩阵金属蛋白酶 (MMP) 活性 (酶谱).

主要成果:

ZrO2表现出四角形的晶体结构和表面形态,支持细胞粘附.
ZrO2调节的细胞粘附 (下调的Src) 和增殖 (上调的p15,CDK2,CDK4).
在不同的机械条件下对MMP2/MMP9活性和TGF-β/MAPK信号调节的不同影响.

结论:

稳定 (ZrO2) 对内皮细胞增殖和ECM重塑有积极的影响.
ZrO2 影响关键信号通路 (Src,TGF-β,MAPK),影响血管生成和细胞动态.
在牙科植入物中,ZrO2显示了增强血管化和组织集成的潜力.

关键词:

细胞循环规则细胞循环规则内皮细胞内皮细胞细胞外矩阵重塑细胞外矩阵重塑剪切压力剪切压力稳定的稳定.

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