Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Chatbot language style matching and reuse intention in service failure: A communication accommodation theory explanation.

Acta psychologica·2026

Same author

Efficacy and safety of allogeneic umbilical cord blood mononuclear cells therapy in children with autism spectrum disorder and immune dysregulation: a single-center, double-blinded, randomized, placebo-controlled trial.

Stem cell research & therapy·2026

Same author

Discovering putative novel stem rust resistance loci in wheat genetic resources.

The plant genome·2026

Same author

Microbial autotrophy driven by extracellular electrons generated from mechanical forces via flexoelectricity.

Nature communications·2026

Same author

Proband Nanopore Long-Read Genome Sequencing Facilitates Preimplantation Genetic Testing for Facioscapulohumeral Muscular Dystrophy.

Neurology. Genetics·2026

Same author

COST1 stimulates RHD3 GTPase activity to maintain ER morphology and plant growth in <i>Arabidopsis</i>.

Science advances·2026

Search research articles

相关实验视频

Updated: Jun 10, 2025

Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants

Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants

Published on: March 11, 2013

修改后的小鼠脑脊液采集方法

Jiaojiao Wang¹, Zhifang Dong¹, Xiuyu Shi¹

¹Growth, Development, and Mental Health of Children and Adolescence Center, Pediatric Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.

Journal of neuroscience methods

|October 16, 2024

概括

从老鼠幼中收集脑脊液 (CSF) 是一个挑战. 这种新的,可重复的方法允许单个运营商快速提取高质量的CSF,产量高.

关键词:

大脑脊髓液 (CSF) 是一种方法方法方法方法它们是小老鼠.

更多相关视频

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

Published on: March 19, 2018

Author Spotlight: Obtaining High-Quality CSF and Blood Samples for Epilepsy Biomarker Discovery

Author Spotlight: Obtaining High-Quality CSF and Blood Samples for Epilepsy Biomarker Discovery

Published on: September 1, 2023

相关实验视频

Last Updated: Jun 10, 2025

Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants

Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants

Published on: March 11, 2013

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

Published on: March 19, 2018

Author Spotlight: Obtaining High-Quality CSF and Blood Samples for Epilepsy Biomarker Discovery

Author Spotlight: Obtaining High-Quality CSF and Blood Samples for Epilepsy Biomarker Discovery

Published on: September 1, 2023

科学领域:

神经科学是一个神经科学.
生物医学工程生物医学工程

背景情况:

脑脊液 (CSF) 对于诊断大脑疾病和监测治疗疗效至关重要.
从小动物 (如老鼠幼) 收集CSF带来了重大的技术挑战.

研究的目的:

开发一种可靠和可重复的方法来从小鼠中提取CSF.
克服与年轻动物中现有的CSF采集协议相关的困难.

主要方法:

修改了现有的协议,以建立一个新的CSF提取方法,具有精确,可控制的参数.
确保跨研究人员的可复制性和单操作员能力.

主要成果:

新方法允许快速,单操作员的CSF提取.
获得高质量的CSF样本,污染最小 (415nm吸收率<0.05AU).
获得高的CSF产量,从P7幼的33μL到P42幼的51.7μL.

结论:

已经建立了一种可重复的,单操作员的方法,用于从小鼠中快速,高产和高质量的CSF采集.
该协议解决了以前方法的局限性,提供了更好的一致性和效率.