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综合性奥米克揭示了卵细胞生长过程中的代谢模式

Xiang Zhang¹, Juan Ge¹, Yue Wang¹

¹State Key Laboratory of Reproductive Medicine and Offspring Health, Changzhou Maternity and Child Health Care Hospital, Changzhou Medical Center, Nanjing Medical University, Nanjing, China.

Molecular & cellular proteomics : MCP

|October 16, 2024

概括

在生长中的小鼠卵细胞中的新陈代谢显示了增强的糖解和减少的氧化酸化,类似于华堡效应. 在卵细胞发育过程中,核酸和脂肪酸通路也发生了显著的改变.

科学领域:

生殖生物学生殖生物学
代谢学代谢学代谢学
蛋白质组学是指蛋白质组学.

背景情况:

卵细胞质量和胚胎发育取决于受控的新陈代谢.
控制哺乳动物卵细胞生长的特定代谢途径尚不清楚.

研究的目的:

为了全面地绘制老鼠卵细胞在体内生长过程中的时间代谢动态.
识别在发育卵细胞中的新代谢特征和调节机制.

主要方法:

来自小鼠卵细胞的代谢学和蛋白质学数据的综合分析.
在卵细胞生长过程中跟踪代谢变化 in vivo.

主要成果:

生长中的卵细胞表现出增强的糖解和减少的氧化酸化,这是一个类似于沃尔堡的代谢程序.
核酸救援途径被激活,而 de novo 途径被抑制.
脂肪酸合成和酸的道化增加,与减少脂肪酸氧化相吻合.

结论:

建立了小鼠体内卵细胞生长的代谢情景.
确定了关键的代谢转变,包括华堡效应和改变的核酸和脂肪酸代谢.
为未来研究哺乳动物卵细胞代谢和生殖健康提供了基础资源.

关键词:

毛囊毛囊是一个毛囊.代谢过程中的代谢.卵子细胞卵子细胞蛋白质组学蛋白质组学生殖生殖的繁殖.

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