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相关概念视频

Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

15.3K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.3K
DNA-only Transposons02:57

DNA-only Transposons

14.4K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
14.4K
LTR Retrotransposons03:08

LTR Retrotransposons

17.4K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
17.4K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

11.4K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.4K
Epistasis Analysis01:09

Epistasis Analysis

4.9K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
4.9K
Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

14.7K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
14.7K

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相关实验视频

Updated: Jun 10, 2025

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
07:28

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Published on: January 10, 2025

236

使用图谱基因组分析可转换元素插入多态的统一框架.

Cristian Groza1, Xun Chen2, Travis J Wheeler3

  • 1Quantitative Life Sciences, McGill University, Montréal, QC, Canada.

Nature communications
|October 16, 2024
PubMed
概括
此摘要是机器生成的。

格拉菲TE是分析移动DNA插入的新管道,称为可转移元素,这有助于基因组多样性. 这个工具可以帮助研究人员在各种物种和测序类型中轻松研究这些元素.

更多相关视频

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
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Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

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Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity
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Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity

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相关实验视频

Last Updated: Jun 10, 2025

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
07:28

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Published on: January 10, 2025

236
Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
10:08

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis

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Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity
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Real-Time Quantification of the Effects of IS200/IS605 Family-Associated TnpB on Transposon Activity

Published on: January 20, 2023

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科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • 可转移元素 (TE) 是移动DNA序列,通过插入多态化驱动基因组多样性.
  • 分析多态TE对于理解基因组进化和变异至关重要.
  • 现有的方法可能缺乏灵活性或易于使用,以进行全面的TE插入分析.

研究的目的:

  • 引入GraffiTE,一个灵活的计算管道,用于识别和基因定型多态可转换元素插入.
  • 使非专家用户能够对各种物种和数据集的TE景观进行详细分析.
  • 利用图谱基因组和先进的结构变异检测来改进TE分析.

主要方法:

  • 集成最先进的结构变异检测算法与图形基因组方法.
  • 从基因组组合或长读序列数据中识别多态移动元素插入.
  • 使用短读和长读测序数据集对 TE 变异进行基因型定型.

主要成果:

  • 在模拟和真实数据集上的基准测试表明GraffiTE的高精度和回忆率.
  • 格拉菲特成功分析了人类,Drosophila melanogaster,玉米和Cannabis sativa pangenome数据,展示了它的多功能性.
  • 管道揭示了多态TE的详细景观,以及它们在不同个体和品种的频率变化.

结论:

  • 格拉菲TE提供了一个强大的,用户友好的解决方案,用于分析多态可转换元素插入.
  • 该管道与各种测序技术兼容,并且可以适应具有有限 TE 知识的物种.
  • 格拉菲TE促进了对由移动DNA驱动的基因组多样性的全面研究,涉及广泛的生物体.