的替代来源为methanococcus maripaludis及其对酶的进化影响
Devon Payne1, Lisa M Keller1, James Larson2
1Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA.
Communications biology
|October 16, 2024
概括
无氧微生物可以利用除了酸盐以外的其他来源的 (Mo),包括四酸盐和酸盐. 这一发现解决了在古代和现代无氧环境中必不可少的基酶的功能.
科学领域:
- 生物地质化学生物地质化学
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 酶对于全球营养循环 (,碳,硫) 是至关重要的.
- (Mo) 通常来自酸盐,但它在无氧,硫化环境中不可用.
- 在古代和现代无氧息地中Mo的生物可用性呈现出一个重要的科学悖论.
研究的目的:
- 为了研究厌氧生物如何在没有酸盐的环境中获得 (Mo).
- 为了确定其他Mo来源,如四聚酸和化,是否可供无氧微生物生物利用.
- 了解地球氧化之前Mo获取的进化影响.
主要方法:
- 使用一种无氧甲原体模型, * Methanococcus maripaludis * ,用于实验性生长研究.
- 使用Mo-依赖酶培养生物体:甲酸脱酶,甲基甲氨酸脱酶和酶.
- 在不同Mo来源 (molybdate,tetrathiomolybdate,molybdenite) 中比较了Mo运输和辅因子生物合成蛋白的生长动力学,Mo含量和基因表达.
主要成果:
- *Methanococcus maripaludis*成功地利用了四聚酸盐和化来满足其高Mo的需求.
- 增长动力学和细胞Mo含量与用酸盐,四聚酸盐和丁酸盐培养的细胞之间是可比的.
- 在所有测试的Mo来源中,Mo运输和辅因子生物合成蛋白的转录水平相似,这表明保留了利用途径.
结论:
- 甲和卜丁是无氧微生物的可生物利用的Mo来源.
- 这项研究解决了Mo在无氧环境中获得Mo的悖论,无论是现代还是古代.
- 这些发现提供了关于基酶在广泛氧化之前的早期演变的见解.
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